Sequences from confirmation amplicon


Confirmation sequences for line 009G02 and hit At2g27820 are shown.
Line ID 009G02
Locus At2g27820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:11857787
Primers used for PCR EV14 - 8474, show primer details
   
Sequence Name 01-K104193-0022-009-G02C-EV14
GenBank Accession LN486554 [GenBank]
Sequencing primer gene specific primer, EV14: 5'-TATCCTAACTGTCAAGCGATTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104193-0022-009-G02C-EV14
GTTCAGCGGTTGAGCTTTGGATCGCCGATCGCGCTGTTTACCCGTCGAAAACTCACTCGG
CGGTTCGATTCATCGTAATTACGATCTTCTCCTCCGTCACCGTCTTCACATCGTCGGCGA
AGTTCAGCTTCCGGTTCATCATTGTCTCATTGCTCTTCCCGGTGTACGGAAAGAGTTTCT
CACTCGTGTGATCTCTCATCCTCAAGGACTAGCTCAATGCGAACACACGTTGACTAAACT
CGGTCTCAACGTAGCTCGTGAAGCTGTTGATGATACCGCCGGTGCGGCGGAGTTTATCGC
TGCGAATAATATCCGTGACACCGCCGCGATTGCGAGTGCACGCGCGGCGGAGATCTACGG
ATTGGAGATTTTGGAAGATGGGATACAAGATGACGCGAGTAACGTCACGCGCTTTGTGAT
GTTGGCGCGTGAACCGATNATACCGAGAACTGATCGGCCGTTTAAGACGAGTATAGTGTT
TGCTCACGAGAAAGGCACGTGCGTTTTGTTTAATGGCGAACCATTAGAATATATTGGTAA
ATCTACATGTAAAAATATGGGTAAACCTGACGTCTACCCATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCA
   
Sequence Name 17-K104194-0022-009-G02C-8409
GenBank Accession LN493830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104194-0022-009-G02C-8409
TTCGGACTGAGCCATTTACATTGAATAATGGGTAGACGTCAGGTTTACCCATATTTTTAC
ATGTAGATTTACCAATATATTCTAATGGTTCGCCATTAAACAAAACGCACGTGCCTTTCT
CGTGAGCAAACACTATACTCGTCTTAAACGGCCGATCAGTTCTCGGTATAATCGGTTCAC
GCGCCAACATCACAAAGCGCGTGACGTTACTCGCGTCATCTTGTATCCCATCTTCCAAAA
TCTCCAATCCGTAGATCTCCGCCGCGCGTGCACTCGCAATCGCGGCGGTGTCACGGATAT
TATTCGCAGCGATAAACTCCGCCGCACCGGCGGTATCATCAACAGCTTCACGAGCTACGT
TGAGACCGAGTTTAGTCAACGTGTGTTCGCATTGAGCTAGTCCTTGAGGATGAGAGATCA
CACGAGTGAGAAACTCTTTCCGTACACCGGGAAGAGCAATGAGACAATGATGAACCGGAA
GCTGAACTTCGCCGACGATGTGAAGACGGTGACGGAGGAGAAGATCGTAATTACGATGAA
TCGAACCGCCAAGTGAGTTTTCGACGGGTAAAACAGCGCGATCGGCGATCCAAAGCTCAA
CCGCTTGAAACGCAACTTCGAATTGATCGCAAGGAATCGCTT

Last Updated on 06.12.2018 16:11