Sequences from confirmation amplicon


Confirmation sequences for line 009H04 and hit At4g13240 are shown.
Line ID 009H04
Locus At4g13240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7682171
Primers used for PCR MF24 - 8474, show primer details
   
Sequence Name 11-K031691-022-009-H04C-MF24
GenBank Accession LN490995 [GenBank]
Sequencing primer gene specific primer, MF24: 5'-AACGAGAGTTGTTCGAAGAGAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K031691-022-009-H04C-MF24
CTAACAAAGTTCCCTGGTCTTCCCCAATCTTCCCTTGACATTTCCAAAATTCAGTATAAC
AAGGTAATGCTCAAATGTTATTGCTTTATTTTGTTATTCCGATATGATCGGACAAATCAT
CATCTAGGAAAGATTCTGAAAATATTGGTGGTCATGAATCATATATAAATTTTTGATATA
ACATTTTTTAGTAAATTTTAAATAAGATTAACAACACTAACTAACCGTACTTTGGTTGTA
TATTATATGGTGGTTCATTTGTCACTTTATGTGTTCTAACTATAGGATGTGGGACATGCG
GTTTTGGAGAGCTACTCAAGGATATTAGAGAGCTTAGGGTACACGGAAATGTCGAGAATC
GACGATGTACTTTATGCGGATTCTTTAGCCCGGAAGCAATGCACAGGTGAAGAAACATCA
GATGGTGGGAAAATAGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTACATTCA
   
Sequence Name 59-K031691-022-009-H04C-8409
GenBank Accession LN509898 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K031691-022-009-H04C-8409
TATTTTCCACCATCTGATGTTTCTTCACCTGTGCATTGCTTCCGGGCTAAAGAATCCGCA
TAAAGTACATCGTCGATTCTCGACATTTCCGTGTACCCTAAGCTCTCTAATATCCTTGAG
TAGCTCTCCAAAACCGCATGTCCCACATCCTATAGTTAGAACACATAAAGTGACAAATGA
ACCACCATATAATATACAACCAAAGTACGGTTAGTTAGTGTTGTTAATCTTATTTAAAAT
TTACTAAAAAATGTTATATCAAAAATTTATATATGATTCATGACCACCAATATTTTCAGA
ATCTTTCCTAGATGATGATTTGTCCGATCATATCGGAATAACAAAATAAAGCAATAACAT
TTGAGCATTACCTTGTTATACTGAATTTTGGAAATGTCAAGGGAAGATTGGGGAAGACCA
GGGAACTTTTGTTTAAGCAAGACCAAGATCGTCTCGGCTCTCTCTTCGAACAACTCTCG
Primers used for PCR CB37 - 3144, show primer details
   
Sequence Name 19-K105032-0022-009-H04C-CB37
GenBank Accession LN494755 [GenBank]
Sequencing primer gene specific primer, CB37: 5'-GTGGGGGTAACATGTTGTTTTACT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K105032-0022-009-H04C-CB37
AGATATGTTATTGCTAATTGTTTATTCTTTTGCCTAAATATACACACTTATATGGCTTCT
TCAATTGTATCATTTTAGTTGTTCACATGTCTTTTGATTCATCTTCAATGCCTATCTTTA
GGGCTCCTAAAACCATTTAAGTTCTCAAGCTTCTCCAAATATGAGAGTTTCTTTGGCGTC
ATTCTCGGAGATTTTGGAGGTTTCTCTGACTGCCCTTTTGAACTATTGTCACTCCAACCA
ATAAAATCAAGCAATGTCGTCTTTGATGAGTTTTGTGATTCAAGCTTCTCTATTTCCTCT
CCTGAGTAGTTCGAAGATCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGGGCTTA
GATGAGAAACTTCACGATCGATGCCTTGATTTCGCCATTCCCAGATACCCATTTCATCTT
CAGATTGGTCTGAGATTATGCGAAAATATACACTCATATACATAAATACTGACAGTTTGA
GCTACCAATTCAGTGTAGCCCATTACCTTACATAATTCACTCAAATGCTAGGCAGTCTGT
CAACTCGGCGTCAATTTGTCGGCCACTATACGATAGTTGCGCAAATTTTCAAAGTCCTGG
CCTAACATCACACCTCTGTCGGCGGCGGGTCCCATTTGTGATAAATCCACCATCACAATA
GATAGTCTAATGGACGAAAAAAGCGAATATTTCGATGCTG
   
Sequence Name 35-K105033-0022-009-H04C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K105033-0022-009-H04C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGCGAGGACAGGGTACC
AGCTTTGCAATTCATACAGAAGTGAGAAAAATGGCTTCTATGATATCCTCTTCAGCTGTG
ACTACAGTCAGCCGTGCTTCTACGGTGCAATCGGCCGCGGTGGCTCCATTCGGCGGCCTC
AAATCCATGACTGGATTCCCAGTTAAGAAGGTCAACACTGACATTACTTCCATTACAAGC
AATGGTGGAAGAGTAAAGTGCATGGTGACGGTGTTCGGCATTCTGAATCTCACCGAGGAC
TCCTTCTTCGATGAGAGCCGGCGGCTAGACCCCGCCGGCGCTGTCACCGCGGCGATCGAA
ATGCTGCGAGTCGGATCAGACGTCGTGGATGTCGGACCGGCCGCCAGCCATCCGGACGCG
AGGCCTGTATCGCCGGCCGATGAGATCAGACGTATTGCGCCGCTCTTAGACGCCCTGTCC
GATCAGATGCACCGTGTTTCAATCGACAGCTTCCAACCGGAAACCCAGCGCTATGCGCTC
AAGCGCGGCGTGGGCTACCTGAACGATATCCAAGGATTTCCTGACCCTGCGCTCTATCCC
GATATTGCTGAGGCGGACTGCAGGCTGGTGGTTATGCACTCAGCGCAGCGGGATGGCATC
GCCACCCGCACCGGTCACCTTCGACCCGAAGACGCGCTCGACGAGATTGTGCGGTTCTTC
GAGGCGCGGGTTTCCGCCTTGCGACGGAGCGGGGTCGCTGCCGACCGGCTCATCCTCGAT
CCGGGGATGGGATTTTTCTTGAGCCCCGCACCGGAAACATCGCTGCACGTGCTGTCGAAC
CTTCAAAAGCTGAAGTCGGCGTTGGGGCTTCCGCTATTGGTCTCGGTGTCGCGGAAATCC
TTCTTGGGCGCCACCGTTGGCCTTCCTGTAAGGATCTGGGGTCAGCGAGCCTTG

Last Updated on 06.12.2018 16:11