Sequences from confirmation amplicon


Confirmation sequences for line 010G07 and hit At3g05940 are shown.
Line ID 010G07
Locus At3g05940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1778997
Primers used for PCR DC31 - 8474, show primer details
   
Sequence Name 29-K032602-022-010-G07C-8409
GenBank Accession LN499179 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032602-022-010-G07C-8409
ATTACATTGAATATATACGTGTTGCATCCACCACTACCATTAGACGGGTTAGTTTGCAGA
ATAAACCTACATTCTCTGTGCTTATATTAGTGCATTTGCATATATCTTTGATTTCTCCTA
TGATTTTTTTCACAAAATCTTGGAACTTCTTAAATTCTTAACCAAACCATGCCAAGGATC
TTTATGAATGATCATTTGGTTGCATGCACTCTGGGTTAGCAGAAGCATTAGATTATTTTA
GTCCAGATATATCTGTGGGAGTCTTGTTTTCGAGTTCTATGACGACTGCCTTAATTAACA
TGTAGTAACTTATACAAGATTATCTTTTCATATAGGCGTTTTATTAGACGATGCAAGCAA
GGTTGTCTGCAATTTGTTATTCTAAAGCCAATCCTAGTTGCTGTCACACTTGTGCTTTAT
GCAAAAGGGAAATACAAGGATGGAAATTTTAGCCCTGATCAATCCTATCTCTATCTTACC
ATCATCTATACAATATCATACACTGTAGCTCTGTACGCGCTGGTCCTCTTTTATGTGGCA
TGCAAAGATCTGCTTCAGCCATTCAATCCAGTCCCAAAATTTGTGATTATAAAGTCTGTT
GTCTTTCTAACCT
   
Sequence Name 29-K032602-022-010-G07C-DC31
GenBank Accession LN499180 [GenBank]
Sequencing primer gene specific primer, DC31: 5'-GAAAAATCACCTGCCAATAGGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032602-022-010-G07C-DC31
CAATTTTGGGACTGGATTGAATGGCTGAAGCAGATCTTTGCATGCCACATAAAAGAGGAC
CAGCGCGTACAGAGCTACAGTGTATGATATTGTATAGATGATGGTAAGATAGAGATAGGA
TTGATCAGGGCTAAAATTTCCATCCTTGTATTTCCCTTTTGCATAAAGCACAAGTGTGAC
AGCAACTAGGATTGGCTTTAGAATAACAAATTGCAGACAACCTTGCTTGCATCGTCTAAT
AAAACGCCTATATGAAAAGATAATCTTGTATAAGTTACTACATGTTAATTAAGGCAGTCG
TCATAGAACTCGAAAACAAGACTCCCACAGATATATCTGGACTAAAATAATCTAATGCTT
CTGCTAACCCAGAGTGCATGCAACCAAATGATCATTCATAAAGATCCTTGGCATGGTTTG
GTTAAGAATTTAAGAAGTTCCAAGATTTTGTGAAAAAAATCATAGGAGAAATCAAAGATA
TATGCAAATGCACTAATATAAGCACAGAGAATGTAGGTTTATTCTGCAAACTAACCCGTC
TAATGGTAGTGGTGGGATGCAACACGTATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTACT
Primers used for PCR FP39 - 3144, show primer details
   
Sequence Name 75-K035341-022-010-G07C-8409
GenBank Accession LN513184 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K035341-022-010-G07C-8409
ACAAAGCCTTAACCATGAGGTCTGCTTTTGTTGAAACTAAAGTTTAAAATTATGTTCGTC
ATCTATTCCCAAGAGTCAATGAGAACTTAGTATAGTAACTCGAGTAAGTAC
   
Sequence Name 75-K035341-022-010-G07C-FP39
GenBank Accession LN513185 [GenBank]
Sequencing primer gene specific primer, FP39: 5'-AGCAGTTCCAAGAGTTTTCTTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K035341-022-010-G07C-FP39
GTCACTCCATTTAATGTTGAATTCTCTCTGGCTAACATGTTATTGGTTCTTTGGGCATGT
CAGGTTTATGCCTTGATGTCATTCTTGGCTCTCGTTTTGCCCAAGAGCTCTATATATTTT
AACTCTATCCGTGAAGTGTGAGTGTTTCTGATTTTTTCGTATTACTAGTTTGACATGGGA
TGTACTTACTCGAGTTACTATACTAAGTTCTCATTGACTCTTGGGAATAGATGACGAACA
TAATTTTAACCTTTGGTTTCAACAAATGCAGACCTCATGGTTAAGGCTTTGTAATCTTTT
TTTTCTTTTTCTGTTTCAGTTACGAAGCATGGGTGATATATAATTTTCTATCGCTGTGTT
TGGCCTGGGTTGGAGGCCCAGGTTCGGTAGTTATAAGTTTCCCATGGAGTCAAAGATTCA
AATAGAGGACCTAACAGAACTCGCCGTAAAGACTGGCGAACAGTTCATACAGAGTCTCTT
ACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACGCTTGTCTA
CTCCAAAAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCAATTGAGACTTTTTCACA
AAGGGTAATATCCCGGAAACTCCTCGGATTCCATTGCC

Last Updated on 06.12.2018 16:11