Sequences from confirmation amplicon


Confirmation sequences for line 010H02 and hit At1g78130 are shown.
Line ID 010H02
Locus At1g78130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29401271
Primers used for PCR GL04 - 8474, show primer details
   
Sequence Name 24-K016380-022-010-H02C-8409
GenBank Accession LN496785 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K016380-022-010-H02C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGGTAATTACTCTTTCTTTTTTCTTGGAA
TGCTCCTGCTACCAACAAGTAAAGTTTCTCTCTTTACTATGCCTAAAGACTTTATATCTC
TCTATCGTTTAACTGTTTTTGCTGACCTGGTGAAACAAACGCAGCCCAATCTTCGCAGAG
ATCGTTCCAGAGAAATCAAGAACAAGCGTCTACGCACTGGACAAATCGTTTGAGTCTATC
TTGTCATCGTTTGCACCTCCTATAGTTGGAATCCTGGCACAACATGTTTATGGATACAAG
CCAATCCCTGAAGGGTCATCGAGGTCAACAGAGATAGCCACAG
   
Sequence Name 28-K016380-022-010-H02C-GL04
GenBank Accession LN498627 [GenBank]
Sequencing primer gene specific primer, GL04: 5'-ATCTGTGGCTATCTCTGTTGACCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K016380-022-010-H02C-gl04
CCTTCAGGATTGGCTTGTATCCATAAACATGTTGTGCCAGGATTCCAACTATAGGAGGTG
CAAACGATGACAAGATAGACTCAAACGATTTGTCCAGTGCGTAGACGCTTGTTCTTGATT
TCTCTGGAACGATCTCTGCGAAGATTGGGCTGCGTTTGTTTCACCAGGTCAGCAAAAACA
GTTAAACGATAGAGAGATATAAAGTCTTTAGGCATAGTAAAGAGAGAAACTTTACTTGTT
GGTAGCAGGAGCATTCCAAGAAAAAAGAAAGAGTAATTACCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11