Sequences from confirmation amplicon


Confirmation sequences for line 011F01 and hit At5g05180 are shown.
Line ID 011F01
Locus At5g05180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:1535948
Primers used for PCR EV19 - 8474, show primer details
   
Sequence Name 75-K037328-022-011-F01C-EV19
GenBank Accession LN513191 [GenBank]
Sequencing primer gene specific primer, EV19: 5'-AGGGAACAAAAGAGCAGATTTACA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K037328-022-011-F01C-EV19
AGGAAGGTTTAAGTAGAGGATTGCTAGGGTACGAGAGTTACGAAGCAGAAGGTGGAAAGG
TTTAAAGGGACGACAAAGAGGAGGGAAGATTTGTCGGATAGCGAAGAGAAAGTGGCGCTC
TTTGGGATTCAAATGTTTGCCATTTTCTTCTTTTGTCTATCTGACAGTCTGTTCTTTGCT
GCACTAGTGGCCACACATCTCCACATGCACCACCAGTTATCAAAGTTTGACCTATTAATT
ATTGGGTTCGGTTCGATTCAATTTTCTCTCGTAAATAACGTAAACCGAACATATACCAAT
TTTGTAGCTTGGCTGCAGGTCGACCTGCAGGCATGCCCGCTGAAATCACCAGTCTCTCTC
TACAAATCTATCTCTCTCTATAATAATGTGTGAGTAGTTCCCAGATAAGGGAATTAGGGT
TCTTATAGGGTTTCGCTCATGTGTTGAGCATATAAGAAACCCTTAGTATGTATTTGTATT
TGTAAAATACTTCTATCAATAAAATTTCTAATTCCTAAAACCAAAATCCAGGGGTACCGA
GCTCGGTACCCGGGGATCAGATCTTATAATTAAATGGCCTTCGCTGCCCATATTATTGGT
AACTCAACAGCATCAATCACGGGATTTTTCTCGAATTAATTGCGTCGAATCTCAGCATCG
AAATATTCGCCTTTTTCGTCCATTAGACTATCTATTGTGATGGTGGATTTATCACAAATG
GGACCCGCCGCCGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAACAAATTAT
   
Sequence Name 82-K037328-022-011-F01C-oril
GenBank Accession LN514148 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K037328-022-011-F01C-oril
GAGCCATTTCAATTGAATATATCCTGTCGGCGGCGGGTCCCATTTGTGATAAATCCACCA
TCACAATAGATAGTCTAATGGACGAAAAAGGCGAATATTTCGATGCTGAGATTCGACGCA
ATTAATTCGAGAAAAATCCCGTGATTGATGCTGTTGAGTTACCAATAATATGGGCAGCGA
AGGCCATTTAATTATAAGATCTGATCCCCGGGTACCGAGCTCGGTACCCCTGGATTTTGG
TTTTAGGAATTAGAAATTTTATTGATAGAAGTATTTTACAAATACAAATACATACTAAGG
GTTTCTTATATGCTCAACACATGAGCGAAACCCTATAAGAACCCTAATTCCCTTATCTGG
GAACTACTCACACATTATTATAGAGAGAGATAGATTTGTAGAGAGAGACTGGTGATTTCA
GCGGGCATGCCTGCAGGTCGACCTGCAGCCAAGCTACAAAATTGGTATATGTTCGGTTTA
CGTTATTTACGAGAGAAAATTGAATCGAACCGAACCCAATAATTAATAGGTCAAACTTTG
ATAACTGGTGGTGCATGTGGAGATGTGTGGCCACTAGTGCAGCAAAGAACAGACTGTCAG
ATAGACAAAAGAAGAAAATGGCAAACATTTGAATCCCAAAGAGCGCCACTTTCTCTTCGC
TATCCGACAAATCTTCCCTCCTCTTTGTCGTCCCTTTAAACCTTTCCACCTTCTGCTTCG
TAACTCTCGTACCCTAGCAATCCTCTACTTAAACCTTCCTTTGTTTCTCCTTCACTTTTC
CTGTAAATCTG

Last Updated on 06.12.2018 16:11