Sequences from confirmation amplicon


Confirmation sequences for line 011F07 and hit At1g23310 are shown.
Line ID 011F07
Locus At1g23310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8268938
Primers used for PCR BJ03 - 3144, show primer details
   
Sequence Name 35-K104374-0022-011-F07C-BJ03
GenBank Accession LN501821 [GenBank]
Sequencing primer gene specific primer, BJ03: 5'-ATTACAAATTTCACATTGCCACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K104374-0022-011-F07C-BJ03
TCTATGGCCTAAACTTCATTTCATTGAGAAAAGATCTTAATCAGTTTCCAATTGAAAATA
GAAATAGAGACACAAGAGAGAGTTTACACCCAAAAAAAAGAGACACAAAGAGAGAGATAT
GGTTTATAGAATAGGGCAAAGAGAAAGAGTGTACTACAGAGATTTAGCTTTAAAGAGTGT
AGCAGAACACAATAGTCGTTCAGAAGAAGAAGTAATCACATTTTCGAATAACCAAAGTTA
TTATCATACTGAGTCATGAACTCGTCGTTGAACTTCTTGAAGCTATCCATGATCTCCGGC
ATCTCATCTTCTGCTGGCAGGATTGTTGTCCTCAGATGGAACACACTGTCAAAATCCACA
AGTGGAATGAGTTCATAAGAAGAGATCAGATGAGCAAGAAAGAAACAGTAGTAGCAGATT
CTTTACCCTTCTTTTTTTATTTTTGAAGGATTTAACTGTAGTAGTGGATTAAACCGAAGG
CGGGAAAAACAGTAGTAACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCC
CGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGA
AGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 51-K104375-0022-011-F07C-3144
GenBank Accession LN507493 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K104375-0022-011-F07C-3144
CGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGG
GATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTTACTACTGTTTTTCCCGCC
TTCGGTTTAATCCACTACTACAGTTAAATCCTTCAAAAATAAAAAAAGAAGGGTAAAGAA
TCTGCTACTACTGTTTCTTTCTTGCTCATCTGATCTCTTCTTATGAACTCATTCCACTTG
TGGATTTTGACAGTGTGTTCCATCTGAGGACAACAATCCTGCCAGCAGAAGATGAGATGC
CGGAGATCATGGATAGCTTCAAGAAGTTCAACGACGAGTTCATGACTCAGTATGATAATA
ACTTTGGTTATTCGAAAATGTGATTACTTCTTCTTCTGAACGACTATTGTGTTCTGCTAC
ACTCTTTAAAGCTAAATCTCTGTAGTACACTCTTTCTCTTTGCCCTATTCTATAAACCAT
ATCTCTCTCTTTGTGTCTCTTTTTTTTGGGTGTAAACTCTCTCTTGTGTCTCTATTTCTA
TTTTCAATTGGAAACTGATTAAGATCTTTTCTCAATGAAATGAAGTTTAGGCCATAGATT
ATTTTTTTTAATCAACGGTGATAGCCTTTTTTTAGTGTGGCAATGT

Last Updated on 06.12.2018 16:11