Sequences from confirmation amplicon


Confirmation sequences for line 012H09 and hit At5g52260 are shown.
Line ID 012H09
Locus At5g52260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21219950
Primers used for PCR M19a - 8474, show primer details
   
Sequence Name 14-K014432-0022-012-H09C-M19a
GenBank Accession LN492276 [GenBank]
Sequencing primer gene specific primer, M19a: 5'-ATTTTGCAATCCGAGACCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K014432-0022-012-H09C-M19a
TAATCAGACCCAAATCATAAAAATCAAATGAAAAATCTACTTAATGAATGGTTTTTCACA
TAGACCTGAATGGATTGTAGACAACAAAAATAGAAGGTGTATTTTTTATTAGGGTTTCTT
ACTTGTTACCCAAGGAAGAATGTAAAGTCAAGATGGTCTCTTCTTCTTCTTCACTAAACG
ACCCCCTCTTTATTCCTGGTCTTAGGTAATTAATCCACCTTAATCTGCAGCTTTTCCCAT
TCCTTTGCAATCCTGGCACAACAATTACTCTTAGTTAAGGATAAGATTAACAACAATGAT
GAGAATTAAGAGTCTAATATAGGATATAGGGTTAAGAAAAAAAGAGAGTCTAATATAAGA
TTTTAGAGATTAAAAAAAAACCAGCTAGGATGGGAACAGTGGTCCAGCAAGCATGGCCAC
GAGAGAGGATGAAACTCTTGAGCTTCTGGTCTTCTTCAGGTGACCATAACCCTTTCCTCT
GTCTCTGTTTTGGTCTCTCTCCAGATTTGGTCATCTTCCACGTATCTTTCTTTCTCTCTC
AAGTCGTCTTAATTAAGAGAAACTTGTGTAAATGATGAATTAGAGAGATAGAGAGAGGGA
CAAAGACAGACAGAGAGAAGTATTATGAGCATATATAGATAATTATGCATAGATAGAGTC
ATAGAGAAACCATTATATTAAACGCAAACACAAAAATTACGTTCATAGAAATTATTCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCCATGAGTAT
GATG
   
Sequence Name 13-K014432-0022-012-H09C-8409
GenBank Accession LN491814 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K014432-0022-012-H09C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAATAATTTCT
ATGAACGTAATTTTTGTGTTTGCGTTTAATATAATGGTTTCTCTATGACTCTATCTATGC
ATAATTATCTATATATGCTCATAATACTTCTCTCTGTCTGTCTTTGTCCCTCTCTCTATC
TCTCTAATTCATCATTTACACAAGTTTCTCTTAATTAAGACGACTTGAGAGAGAAAGAAA
GATACGTGGAAGATGACCAAATCTGGAGAGAGACCAAAACAGAGACAGAGGAAAGGGTTA
TGGTCACCTGAAGAAGACCAGAAGCTCAAGAGTTTCATCCTCTCTCGTGGCCATGCTTGC
TGGACCACTGTTCCCATCCTAGCTGGTTTTTTTTTAATCTCTAAAATCTTATATTAGACT
CTCTTTTTTTCTTAACCCTATATCCTATATTAGACTCTTAATTCTCATCATTGTTGTTAA
TCTTATCCTTAACTAAGAGTAATTGTTGTGCCAGGATTGCAAAGGAATGGGAAAAGCTGC
AGATTAAGGTGGATTAATTACCTAAGACCAGGACTAAAGAGGGGGTCGTTTAGTGAAGAA
AAAGAAGAGACCATCTTGACTTTACATTCTTCCTTGGGTAACAAGTAAGAAACCCTAATA
AAAAATACACCTTCTATTTTTGTTGTCTACAATCCATTCAGGTCTATGTGAAAAACCATT
CATTAAGTAGATTTTTCATTTGATTTTTATG
Primers used for PCR 63yl - 8474, show primer details
   
Sequence Name 18-K125592-0022-012-H09C-63yl
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 63yl: 5'-GGCAAATATAAAAAGAAATAACGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K125592-0022-012-H09C-63yl
GGACATATATTTGGAAGTTCACATGAAAAGCCAGCAAAACCCATTTGGGTCACGACATAT
TGTATATGGAAATGCAAAATATTCCCTCTCTCAAATGAATACCCTATTTGTATATTCGAA
ATGTATTGTATACTTCTATTATCAATAAAATCCAAAATTAAGTTTTATACGTAAATATGT
GCAGCAAATAGGAGGGAAGTGTTGACTCACCTACCAAGATTAATTACACAAATATCCTAA
CAAACAAACTATAATATTGATTATATTTAAAGAAATTTTGTGCATAGGGTTTTTAAATAT
ACACATTGCCAACTTTGGTATACGGGATATCCAATTTGTCCGATCGCTTTTCAAAGATTG
GATGATTCTTTGCATTCTTTAATTCAATTGTGTTCATTTTCTTTCATAATCATTGATACG
GGACAGACAAAGATTGTTTTGTCAACGTTAAGTTTTGCCCTCTAGACCACTTTTACTCAT
TTATATGATTTGGTCAAATTACATCATTTACACTTTTACATACACAACATCTTGATATTT
TTACTTTAATATATATTCGGAACCAAACTACTTAAGTCAATTTTTGACATCAATCTGTTT
GACATTGATAGTGTCTTTGAGTGAGTTTTTGTATAAAGAGATGGAGACAAAGTGATACCA
ACTTGGTTAAATCATAGATAATACTGTGATTAATCCTAACATCTTATCATCGTAATGAAG
ATTTCCCTGTGATCGCAAGTTTGTCAATATCTATATNTTATTAGGCGTTAGTCTTTCTTC
ATAGACTTAGAAAAAGAAGAGAACGTANACAAAAGATATCTGAAAGCAAAACAAGCAAAG
ATTCCTTCTT
   
Sequence Name 02-K125591-0022-012-H09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K125591-0022-012-H09C-8409
CCATGTAGACTTTTAGGAATAGCCAAATGGGGCTGCGTCCTATAATAAAAGACACATAAT
TTTATACGTATGCGGACTCAAATGAAATTTCATGTATACACGTGTATGTATGTGTATTTT
GTATTTTGTGCATATGTGTAATTAAGTATATTAGATAGGCTATATAATAAAAGTATGGTA
GAATATGTAAGCAAAAATTAGTTGTAAACCCAAGTCACTTGGTTTAAATAATTAAAGACA
AAAAGAGAAGAAGGAATCTTTGCTTGTTTTGCTTTCAGATATCTTTTGTTTACGTTCTCT
TCTTTTTCTAAGTCTATGAAGAAAGACTAACGCCTAATAAAATATAGATATTGACAAACT
TGCGATCACAGGGAAATCTTCATTACGATGATAAGATGTTAGGATTAATCACAGTATTAT
CTATGATTTAACCAAGTTGGTATCACTTTGTCTCCATCTCTTTATACAAAAACTCACTCA
AAGACACTATCAATGTCAAACAGATTGATGTCAAAAATTGACTTAAGTAGTTTGGTTCCG
AATATATATTAAAGTAAAAATATCAAGATGTTGTGTATGTAAAAGTGTAAATGATGTAAT
TTGACCAAATCATATAAATGAGTAAAAGTGGTCTAGAGGGCAAAACTTAACGTTGACAAA
ACAATCTTTGTCTGTCCCGTATCAATGATTATGAAAGAAAATGAACACAATTGAATTAAA
GAATGCAAAGAATCATCCAATCTTTGAAAAGCGATCGGACAAATTGGATATCCCGTATAC
CAAAGTTGGCAATGTGTATATTTAAAAACCCTATGCACAAAATTTCTTTAAATATAATCA
ATATTATAGTTTGTTTGTTAGGATATTTGTGTAATTAATCTTGGTAAGTGAGTCAACACT
TCCCTCCTATTTGCTGCACATATTTACGTATAAAACTTAATTTTGGATTTTATTG

Last Updated on 06.12.2018 16:11