Sequences from confirmation amplicon


Confirmation sequences for line 013E08 and hit F11C10 are shown.
Line ID 013E08
Locus F11C10 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19090976
Primers used for PCR H17b - 3144, show primer details
   
Sequence Name 61-K025676-022-013-E08C-3144
GenBank Accession LN510414 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K025676-022-013-E08C-3144
TAGGGATGACATCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTT
CATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTT
TAAACTATCAGTGTTTCCCATATATGGTTGCTGATCATATTGACCATCATACTCATTGCT
GATATCTCCATTTACAAGTGGATCCATGTGGATTTCCCGGACATATTTGTAAGCAAGTGG
ATTTATTCAGTTACAATTTGGGATCCATTCTTTTTTTTTTTCAAATAAAGCAATAGTTAT
AACAAGTTAATATTATCCAATCAATGTTATTCAGGAAAATATTTGATTAATTATCTTGAA
AAAGGTTCATGTACATTTGAATTTTGAAGTGACCAAATCTTATATATGGGTCATGAATAT
GATATATTGACATTTCAAGAGACTGAGCTAAAAATTACTTAAAAATGAGAATCAATTATT
TTTTTTCCATTGGCCCATTATTTTATGCAATGCAGCACATAAAAATCTATTTTGGTTTTG
GTTTGGGCTTCTGGTAGTTCTGAAAAGCCCAGTATAAAGCCCAACTCCATTAATGCATCT
TCACCTCT
   
Sequence Name 62-K025676-022-013-E08C-H17b
GenBank Accession LN510651 [GenBank]
Sequencing primer gene specific primer, H17b: 5'-AGATAATCTGACGGAGAAGGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K025676-022-013-E08C-h176
GGGGATGAACATTGGATCTGATCGGAGAAACAAACCCTATGTGAAATGAATCGGAAGCAC
AATTCTCGTCGTAGAGGTGAAGATGCATTAATGGAGTTGGGCTTTATACTGGGCTTTTCA
GAACTACCAGAAGCCCAAACCAAAACCAAAATAGATTATTATGTGCTGCATTGCATAAAA
TAATGGACCAATGGAAAAAAAATAATTGATTCTCATTTTTAAGTAATTTTTAGCTCAGTC
TCTTGAAATGTCAATATATCATATTCATGACCAATATATAAGATTTGGTCACTTCAAAAT
TCAAATGTACATGAACATTCTACAAGATAATTAATCAAATATTTACCTGAATAACATTGA
TTGGATAATATTAACTTGTTATAACTATTGCTTTATTTGAAAAAAAAAAAGAAGGGATCC
CAAATTGTAACTGAATAAATCCACTTGCTTACAAATATGTCCGGGAAATCCACATGGATC
CACTTGTAAATGGAGATATCAGCAATGAGTATGATGGTCAATATGATCAGCAACCATATA
TGGGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCC
TGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAA
Primers used for PCR 35wl - 8474, show primer details
   
Sequence Name 41-K118446-0022-013-E08C-35wl
GenBank Accession LN504257 [GenBank]
Sequencing primer gene specific primer, 35wl: 5'-ACGCAATTTCTTACATCAAAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118446-0022-013-E08C-35wl
GAGTAGGAACAGATAGAGCTTATCAGAATCAAACACAATAACAGTAGAAGAAAGTCCAGA
AGTTGACATTCAAGCTATGAATGAAGAGGTTGTTGTAAGAGTAATCTCGCCTTTGGATTC
ACATCCAGCTTCAAGAATCATACAAGCAATGAGAAACTCAAATGTTAGTCTAATGGAGGC
TAAGTTATCATTAGCTGAAGACACAATGTTTCACACTTTTGTGATAAAGTCTAACAACGG
GTCGGATCCATTGACGAAAGAGAAGCTTATAGCAGCGTTTTACCCCGAGACCAGCTCGAC
GCAACCGCCATTGCCTTCTTCTAGTTCACAGGTCTCTGGTGATATATAAGTTTCACATGA
TGGTGAAGCTTTTGTCTATTCTTGTTTCAGTATAGATAACTTTCAGGTTCAACTAGAAAT
AGATAAAATCCTTTTTTCTTTTCTCTTTCTTATAGTGAAAGGAATCCGGTTTAAGTATAT
TCATTAACTTTATTTAGACCATTGATTCACATATTTGTATCTTGGGAAAATATTTCCTAT
ACTTTAGAAAATCAAATAAAATATTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 01-K118445-0022-013-E08C-8409
GenBank Accession LN486782 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118445-0022-013-E08C-8409
CATTGAATATATCCTGAATATTTTATTTGATTTTCTAAAGTATAGGAAATATTTTCCCAA
GATACAAATATGTGAATCAATGGTCTAAATAAAGTTAATGAATATACTTAAACCGGATTC
CTTTCACTATAAGAAAGAGAAAAGAAAAAAGGATTTTATCTATTTCTAGTTGAACCTGAA
AGTTATCTATACTGAAACAAGAATAGACAAAAGCTTCACCATCATGTGAAACTTATATAT
CACCAGAGACCTGTGAACTAGAAGAAGGCAATGGCGGTTGCGTCGAGCTGGTCTCGGGGT
AAAACGCTGCTATAAGCTTCTCTTTCGTCAATGGATCCGACCCGTTGTTAGACTTTATCA
CAAAAGTGTGAAACATTGTGTCTTCAGCTAATGATAACTTAGCCTCCATTAGACTAACAT
TTGAGTTTCTCATTGCTTGTATGATTCTTGAAGCTGGATGTGAATCCAAAGGCGAGATTA
CTCTTACAACAACCTCTTCATTCATAGCTTGAATGTCAACTTCTGGACTTTCTTCTACTG
TTATTGTGTTTGATTCTGATAAGCTCTTATCTGTTCCTACTCTTTCATCTTCCATTATCT
TGACTTTCTCTTGAAGCTCTTTGATGTAAG

Last Updated on 06.12.2018 16:11