Sequences from confirmation amplicon


Confirmation sequences for line 015G11 and hit At5g14640 are shown.
Line ID 015G11
Locus At5g14640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4720451
Primers used for PCR JC09 - 8474, show primer details
   
Sequence Name 62-K026684-022-015-G11C-8409
GenBank Accession LN510652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K026684-022-015-G11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCCTTCTGTTCTGGTGTTAG
TTTTCATATTAACTATATTATTTGTTTTATAGTCGTCGAGTCCTAACTATTTTAGCTTTG
CCCCTCTGTAACAGGTTAAAGGCGAGCCAAACATCTCATACATCTGCTCCCGTTACTATC
GAGCACCTGAACTTATATTTGGAGCTACAGAATATACAACAACAATTGACATATGGTCTG
CAGGCTGTGTTCTTGCTGAATTGCTTCTGGGACAGGTAATATGACTGTCCAAACTTTCCT
CCATTTATCTTTTACAAATGATTTGATTTACCGAATGATGTCTAAAGTTATATATTACCA
ATCTTTGGCTTGTCCAACTTTTGGTTAGCCTCTATTTCCAGGTGA
   
Sequence Name 63-K026684-022-015-G11C-JC09
GenBank Accession LN510892 [GenBank]
Sequencing primer gene specific primer, JC09: 5'-ACTCCACTCTCACCTGGAAATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K026684-022-015-G11C-JC09
GCTAACCAAAAGTTGGACAAGCCAAAGATTGGTAATATATAACTTTAGACATCATTCGGT
AAATCAAATCATTTGTAAAAGATAAATGGAGGAAAGTTTGGACAGTCATATTACCTGTCC
CAGAAGCAATTCAGCAAGAACACAGCCTGCAGACCATATGTCAATTGTTGTTGTATATTC
TGTAGCTCCAAATATAAGTTCAGGTGCTCGATAGTAACGGGAGCAGATGTATGAGATGTT
TGGCTCGCCTTTAACCTGTTACAGAGGGGCAAAGCTAAAATAGTTAGGACTCGACGACTA
TAAAACAAATAATATAGTTAATATGAAAACTAACACCAGAACAGAAGGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTTCGACGTGTCTA
C

Last Updated on 06.12.2018 16:11