Sequences from confirmation amplicon


Confirmation sequences for line 016D02 and hit At1g64280 are shown.
Line ID 016D02
Locus At1g64280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:23854446
Primers used for PCR KY09 - 8474, show primer details
   
Sequence Name 04-K036056-022-016-D02C-KY09
GenBank Accession LN487824 [GenBank]
Sequencing primer gene specific primer, KY09: 5'-AGCGCTTTTAGTCTACTTTGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K036056-022-016-D02C-KY09
TTTCCCCGGTGATGTTCTCTTCGTACCAGTGAGACGGTCAGGCTCGAGGCTAGTCACTAT
GAACTCACATGTTCCCTTCATTTCGGCGATCTCCATTGCAGCTTGTGCTTCCGTTGGAAA
AAGACGTTGAGCAAGTGCAACTAAACAGTGGACGACACAAAGAATAGTTATCATTAGTTC
ACTCAGTTTCCTAATAGAGAGGACATAAATTTAATTCAAACATATAAGAAATAAGACTTG
ATAGATACCTCTATTTTCAAGATCGAGCAGCGTCATCTTCAATTCATCGGCCGCCACTGC
AAAAGAGGGAGGAACATCTCTAGGAATTTGTTCTCGTTTGTCTTCTTGCTCTAGTATTTC
TACACATAGTCGGCCTTTGAGAGAATGCTTGCATTGCTCCGGGATATTATTACATTCAAC
CGCCATAGTGGCTTGTTTTGCGATCATGAGTGCGGTTCTACCTTCCAAAGTTGCTTCTGA
TGCACTTGCACCTTTTTCCAATAGAGATAGTATCAATTGTGGCTCCTTCCGCATCGCAGC
AACATGAAGCACCGTATATCCCCTCGGATTCCTATGGTTGACATCGGCNAGATCAAGTTT
TAAAAGATCTGTTGCGGTCTTCACATTGCAATATGCAACAGCGAAATGAAGAGCACACGC
ATCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAANAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTCCGCAGCGTTATTAT
NAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGTCGTAATTATGGGCGAAA
GCAATAAACAAATTATTCTAATTCGGAAATCTGTATNTCGACGTGTCTA
   
Sequence Name 03-K036056-022-016-D02C-8409
GenBank Accession LN487389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K036056-022-016-D02C-8409
GATGCGTGTGCTCTTCATTTCGCTGTTGCATATTGCAATGTGAAGACCGCAACAGATCTT
TTAAAACTTGATCTTGCCGATGTCAACCATAGGAATCCGAGGGGATATACGGTGCTTCAT
GTTGCTGCGATGCGGAAGGAGCCACAATTGATACTATCTCTATTGGAAAAAGGTGCAAGT
GCATCAGAAGCAACTTTGGAAGGTAGAACCGCACTCATGATCGCAAAACAAGCCACTATG
GCGGTTGAATGTAATAATATCCCGGAGCAATGCAAGCATTCTCTCAAAGGCCGACTATGT
GTAGAAATACTAGAGCAAGAAGACAAACGAGAACAAATTCCTAGAGATGTTCCTCCCTCT
TTTGCAGTGGCGGCCGATGAATTGAAGATGACGCTGCTCGATCTTGAAAATAGAGGTATC
TATCAAGTCTTATTTCTTATATGTTTGAATTAAATTTATGTCCTCTCTATTAGGAAACTG
AGTGAACTAATGATAACTATTCTTTGTGTCGTCCACTGTTTAGTTGCACTTGCTCAACGT
CTTTTTCCAACGGAAGCACAAGCTGCAATGGAGATCGCCGAAATGAAGGGAACATGTGAG
TTCATAGTGACTAGCCTCGAGCCTGACCGTCTCACTGGTACGAAGAGAACATCACCGGGT
GTAAAGATAGCACCTTTCAGAATCCTAGAAGAGCATCAAAGTAA

Last Updated on 06.12.2018 16:11