Sequences from confirmation amplicon


Confirmation sequences for line 016H02 and hit At3g62680 are shown.
Line ID 016H02
Locus At3g62680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23182641
Primers used for PCR MT01 - 8474, show primer details
   
Sequence Name 29-K024065-022-016-H02C-8409
GenBank Accession LN499123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K024065-022-016-H02C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATTCAAATTTTTAATACCCCAAA
CATTTTCTTATAAAGCCCCATTTGGTGAAATCGTTTTCCTCATCACAATTTAAATACAAA
GAACACAACAAACGAACTAGCTTAAGGCAATATACAAGCCAAGCGCTCAGCAATGGCGAT
CACACGCTCCTCCTTGGCCATTTGCCTCATCCTCTCTTTGGTTACCATAACCACCGCCGA
TTACTACTCTCCCTCATCTCCTCCGGTTTACAAATCACCGGAACACAAACCCACGCTCCC
GTCTCCGGTTTACACTCCACCCGTTTACAAGCCTACCCTCTCCCCTCCAGTCTACACTAA
ACCCACTATCCCACC
   
Sequence Name 30-K024065-022-016-H02C-MT01
GenBank Accession LN499559 [GenBank]
Sequencing primer gene specific primer, MT01: 5'-AGGTGGGATAGTGGGTTTAGTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K024065-022-016-H02C-MT01
GGGTAGGCTTGTAAACGGGTGGAGTGTAAACCGGAGACGGGAGCGTGGGTTTGTGTTCCG
GTGATTTGTAAACCGGAGGAGATGAGGGAGAGTAGTAATCGGCGGTGGTTATGGTAACCA
AAGAGAGGATGAGGCAAATGGCCAAGGAGGAGCGTGTGATCGCCATTGCTGAGCGCTTGG
CTTGTATATTGCCTTAAGCTAGTTCGTTTGTTGTGTTCTTTGTATTTAAATTGTGATGAG
GAAAACGATTTCACCAAATGGGGCTTTATAAGAAAATGTTTGGGGTATTAAAAATTTGAA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC

Last Updated on 06.12.2018 16:11