Sequences from confirmation amplicon


Confirmation sequences for line 016H10 and hit At5g01900 are shown.
Line ID 016H10
Locus At5g01900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:351501
Primers used for PCR IS16 - 8474, show primer details
   
Sequence Name 11-K016201-022-016-H10C-8409
GenBank Accession LN490894 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K016201-022-016-H10C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGCAGGTTCAAAAGATCCAAC
ACAATCCTCCATTGTACTCAACCACTTACTTCGGCCAGCACATATGCCAGCTTCATCAAG
CTTATGCAACTTTCCCCATTGACACATCTGACTTCGAAGAGCACGAGGGATCCCACATGA
TCCGATTCGGTCACCCCAACATCAGCTTCTCCTCCTCCACCTCTAACCTCCGCCAACATC
AGAATCATCAGGATCGCATCAAAGATGAATACATGAAACCGGTGATAGCAGAAGATTGGT
CTCCATCCCAGTGGATGTCGTCCGAGGTTGCCCTCGCCGTGGAGGCTTTCGAGTTTAATC
CTTTCTGGACATCTCACGACTTATCATCATGACACTATATCCCTCAGCTCTATCATCATA
CATATATAGCTTCTTTAATCCTAAAAACCTCTATATATATATATAGTCTTATACATCTCC
ATGTAATATGTTTCAATCTCTACTTGGTGATTTTGTTTGAGTACTCATCATATTAACCTA
CAATAATACATGAAGGTTGTACTGTTGTTGCCAGCTTTTCCAATTTTATACATACGGGAA
TCTTTCATAAACAAACCTTTGAGTAAAGTTTTCTAGTACCTTTCAACCATCAGCGGACAT
TTATGTGATAATATATGTATACTACTCTTT
   
Sequence Name 12-K016201-022-016-H10C-IS16
GenBank Accession LN491350 [GenBank]
Sequencing primer gene specific primer, IS16: 5'-CTCATGCTTAAATGCGTACCATAA-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K016201-022-016-H10C-is16
GCCACTTGCAAAGAATGATTGTTTCTCTGGATTCAAACATTGGGTACTAGCTAGGTACTG
TTCAATGTAGAATAAATCCACCAAGTCTAGATCTGCTTCAACGTCACCCTCTTTTCTTAT
AAAAGAGTAGTATACATATATTATCACATAAATGTCCGCTGATGGTTGAAAGGTACTAGA
AAACTTTACTCAAAGGTTTGTTTATGAAAGATTCCCGTATGTATAAAATTGGAAAAGCTG
GCAACAACAGTACAACCTTCATGTATTATTGTAGGTTAATATGATGAGTACTCAAACAAA
ATCACCAAGTAGAGATTGAAACATATTACATGGAGATGTATAAGACTATATATATATATA
GAGGTTTTTAGGATTAAAGAAGCTATATATGTATGATGATAGAGCTGAGGGATATAGTGT
CATGATGATAAGTCGTGAGATGTCCAGAAAGGATTAAACTCGAAAGCCTCCACGGCGAGG
GCAACCTCGGACGACATCCACTGGGATGGAGACCAATCTTCTGCTATCACCGGTTTCATG
TATTCATCTTTGATGCGATCCTGATGATTCTGATGTTGGCG
Primers used for PCR FM76 - 3144, show primer details
   
Sequence Name 47-K029301-022-016-H10C-3144
GenBank Accession LN506091 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K029301-022-016-H10C-3144
CTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGG
TACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAATGGCTTCTCTTATTCTTTT
GTTTTTAAGCCATTTGTGTGGTTGAGTACAATGGAGGATTGTAATGGAGTTCTGGTCCTT
GGTGTAGGCACACTTGTAGTAACTCCTTTGGTACTCAGATTCTTTGATCTGCTTTTGTCC
GTATTTCCTCCAGAGGAAGCCGTCGTGGTAGATCGGGGTTGAAGATTCGTGTCTATGGTA
GTCTGAGCTCTCTGCCATAGATGTCTT
   
Sequence Name 48-K029301-022-016-H10C-FM76
GenBank Accession LN506404 [GenBank]
Sequencing primer gene specific primer, FM76: 5'-AGAAAGACATCTATGGCAGAGAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K029301-022-016-H10C-FM76
TCACCCCGAATCTACCACGAACGGCTTCCCTCTGGAGGAAATACGGACAAAAGCAGAATC
AAAGAATCTGAGTACCAAAGGAGTTACTACAAGTGTGCCTACACCAAGGACCAGAACTCC
ATTACAATCCTCCATTGTACTCAACCACACAAATGGCTTTAAAAACAAAAGAATAAGAGA
AGCCATTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCA
AATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGT
CATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAA
CGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGG
Primers used for PCR 58wf - 3144, show primer details
   
Sequence Name 15-K118238-0022-016-H10C-58wf
GenBank Accession LN493173 [GenBank]
Sequencing primer gene specific primer, 58wf: 5'-CATCCGATAGAGAGGTTAACGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K118238-0022-016-H10C-58wf
CATTATCTCGGTCTTCTCGACCTATTTTTATATATATCTAAAGAGAGAGAGACACAATGG
ATCACATCTCTAAACTTAATAAACCCACAGAGCAATAATTATAAGTATTTACTCAAAAAA
AAACAAAAGACGATGAACTCTTGCCAACAAAAGGCTATGGAGAAGTTGCTTCACGGGCAT
GGGTGCGCCAACCAACTGCTCATCATGGACCAGACCGAGTCAGACTCGTCCATGGAAAGG
GAGGATTTGGCCAAGTCCGTCCTCCATTGTTTCTCAGATGCGCTCTCCATCTTGATCGAT
ACTAATGACCACCAAGACGATCAATCCAATAACTCATCTCCTCAGGACTCTACTCCTGTG
CTTGAGAGCTCCAGGAAACCACTTCACATGAGGGGAAGGTTTGAATATAAACATCGTATT
CATAAATTGAAAATGTTCTATCTTTGAGTGATTGCTCACCAGCGGGAATCTCAGGTGGTT
GCAGAAAGACATCTATGGCAAAGAGCTCAAACAACCATAGACACGAATCTTCAACCCCGA
TCTACCCCGACGG
   
Sequence Name 01-K118237-0022-016-H10C-35St
GenBank Accession LN486774 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K118237-0022-016-H10C-35St
CAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGAT
CAGATTGTCGTTTCCCGCCTTCGGTTTAATGGCTTCTCTTATTCTTTTGTTTTTAAGCCA
TTTGTGTGGTTGAGTACAATGGAGGATTGTAATGGAGTTCTGGTCCTTGGTGTAGGCACA
CTTGTAGTAACTCCTTTGGTACTCAGATTCTTTGATCTGCTTTTGTCCGTATTTCCTCCA
GAGGAAGCCGTCGTGGTAGATCGGGGTTGAAGATTCGTGTCTATGGTAGTCTGAGCTCTC
TGCCATAGATGTCTTTCTGCAACCACATGAGATTCATGATGGTGAGCAATCACTCAAAGA
TAGAACATTTTCAATTTATGAATATGATGTTTATATTCATACCTTCCCCTCTTGTGAAGT
GGTTTCCTGGAGCTCTCAAGCACAGGAGAAGAGTCCTGAGGAGATGAGTTATTGGATTGA
TCGTCTTGGTGGTCATTAGTATCGATCAAGATGGAGAGCGCATCTGAGAAACAATGGAGG
ACGGACTTGGCCAAATCCTCCCTTTCCATGGACGAGTCTGACTCGGTCTGGTCCATGATG
AGCAGCTGGTTGGCGCACCCATGCCCGTGAAGCAACTTCTCCATAGCCTTTTGTTGGCAA
GAGTTCATCTNTTTTTGTTTTTTTTTGAGTAAATACTTATAATTATTGCTCTGTGGGTTT
ATTAAGTTTAGAGATGTGATCCATTGTGTCTCTCTCTCTNTAGATATATATACGAATAGG
TCGAGAAGACGAGATAATGGTCTTGTTACGAAGTTTCGTACGTGTGAGAGAGTTTAGTCA
ATTACTATACACTCCTTCGTTAAC

Last Updated on 06.12.2018 16:11