Sequences from confirmation amplicon


Confirmation sequences for line 017A01 and hit At1g74700 are shown.
Line ID 017A01
Locus At1g74700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28066743
Primers used for PCR ME01 - 8474, show primer details
   
Sequence Name 14-K021851-022-017-A01C-ME01
GenBank Accession LN492314 [GenBank]
Sequencing primer gene specific primer, ME01: 5'-GTGTTGAGGTTTGTCTTTGATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K021851-022-017-A01C-ME01
TTTTTTCTATGTTTGTCTATCACAAAGTCAATAGTTTTGCAATGGGTCTTTCCAAAGTAT
ATAAGCCTGTTTATAAGTTTGGATTGTGGATTGTAGATTACAGACAGCATAATAACTCCT
GAAGTTGCTTTTACGGGAGATACAACGTCGGATTTTGTAGTTGATGAAACTAATGCTGAT
GCTCTCAAGGCAAAGGTTCTCGTCATGGAGGTGAATATCTGCTACTGATATGAATTTGGA
CAATGTGCGTGATTTATAATGATATAGTTCTTCCCGGTCCAAGTTTCATGGCTTTGTATT
AAAAGGGAAGCTGTCTCTATTTCCTCTTGTGCAGAGCATATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT
   
Sequence Name 13-K021851-022-017-A01C-8409
GenBank Accession LN491845 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K021851-022-017-A01C-8409
TCGGATGAGCCTTTACATTGAAATATGCTCTGCACAAGAAGGAAATNAGAGACACGCTCT
CCCTTTTAATACAAAGCCATGAAACTTGGACCGGGAAGAACTATATCATTATAAATCACG
CACATTGTCCAAATTCATATCAGTAGCAGATATTCACCTCCATGACGAGAACCTTTGCCT
TGAGAGCATCAGCATTAGTTTCATCAACTACAAAATCCGACGTTGTATCTCCCGTAAAAG
CAACTTCAGGAGTTATTATGCTGTCTGTAATCTACAATCCACAATCCAAACTTATTAAAC
AGGCTTATATACTTTGGAAAGACCATTGCAAAACTATTGACTTTGTGATAGACAAACATA
AGAAAAAATTGACAAGCAATATTAAGCAAATCAAAGACAAACCCTCAAC
Primers used for PCR FL28 - 3144, show primer details
   
Sequence Name 95-K028754-022-017-A01C-3144
GenBank Accession LN515322 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K028754-022-017-A01C-3144
CCCGGGGAATCGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGCGTTTGAGATTCGG
TATCGGTAGTGCATGCGAGAGATTATGGACATATCCATATATCTGAGGTCAGGATCCTCC
TTTCTTTGACACCTCTTGTTACTTTCTGTCTCTTGATGGTCATAGAAGATTTCATATATC
CTTAAACTGCAGATAGTAAATCATGCTGAAAAGTTTGAAAACAAAGCAATCCTGCTAATC
CACTTTTCGGCTCGGTATACAGTGAAGGTGTGTCTTCTTTATCCAACCTGGTTATGAAGC
AGTAGGAAATCAGTTACTCTCTTACCTTGTAGTGGTAATTAATCTTTGCGAGTTTTCACA
GGAAATCGAAGATGCGGTTTCTGCATTGCCTCCACCTTTAGAGGGACGTGTGTTTGCACT
AACACAAGGATTCTAAACATTATAACACTCTTATAGGTTTTACATACTTTTGTTTTTGTA
TTCCACATGTAAACATTGTATTCTGTTGTTAATTTTAAGATTTCTTCTATCTCGATAAAG
AATGAAGTGTGGAAGTAGAGAATACAGACGAGCACACCAATATCAGATAAATTCAGAGGA
AATCTCATGGGATCCCAAATC
   
Sequence Name 95-K028754-022-017-A01C-FL28
GenBank Accession LN515323 [GenBank]
Sequencing primer gene specific primer, FL28: 5'-TTGCAGGGAAATAATAAGATTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K028754-022-017-A01C-FL28
GCTCGTCTGTATTCTCTACTTCACACTTCATTCTTTATCGAGATAGAAGAAATCTTAAAA
TTAACAACAGAATACAATGTTTACATGTGGAATACAAAAACAAAAGTATGTAAAACCTAT
AAGAGTGTTATAATGTTTAGAATCCTTGTGTTAGTGCAAACACACGTCCCTCTAAAGGTG
GAGGCAATGCAGAAACCGCATCTTCGATTTCCTGTGAAAACTCGCAAAGATTAATTACCA
CTACAAGGTAAGAGAGTAACTGATTTCCTACTGCTTCATAACCAGGTTGGATAAAGAAGA
CACACCTTCACTGTATACCGAGCCGAAAAGTGGATTAGCAGGATTGCTTTGTTTTCAAAC
TTTTCAGCATGATTTACTATCTGCAGTTTAAGGATATATGAAATCTTCTATGACCATCAA
GAGACAGAAAGTAACAAGAGGTGTCAAAGAAAGGAGGATCCTGACCTCAGATATATGGAT
ATGTCCATAATCTCTCGCATGCTCTACCGATACCGAATCTCAAACACTGATAGTTTAAAC
CGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAAC
TTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGNGATTGTGCGTCATCCCTTACGT
CAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTT
CCACGATGCTCCTCG

Last Updated on 06.12.2018 16:11