Sequences from confirmation amplicon


Confirmation sequences for line 017C05 and hit At2g31865 are shown.
Line ID 017C05
Locus At2g31865 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13547368
Primers used for PCR EP09 - 8474, show primer details
   
Sequence Name 08-K102173-0022-017-C05C-8409
GenBank Accession LN489679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K102173-0022-017-C05C-8409
TTACAATTGAATATATCCTGTAAATTTAATCTTGGTCTAGTCAAGAAAAACAAAATGCTT
AACATGTATGCTAAATTCATGTATGGAGCATAATTGTGGTCTTACCGAAGATGATGCCTC
AGGATCATGCTTAACATCGATATTATATTTACATTGATGCATGTATCCGCTAAAAGCCTT
ATTAACCTCTCTACAGAAACAAAAGGAGCAATTGAGTAATTCACAAACAAAATAAAAAAA
AGGAAAGAATGAAAAACTGGCTGAAAGACAAGTTTTGATTACCGAATGAGGGCATCAAGT
TTGTACTGTCCCATTCCTGGGTCAGGCATGGCATCTATAGCTATGACTCTTGTTTTTCGC
CTCCTGAAAATGTCTAAGTCCTTGTTGTCTGTGTAATCACCAGCATATTGGAACGAAGGC
CCATACCTGAATGGAGCCACCATGAGTTGGATTTAGAGAGAAGACTATATAATAAGCTTA
ATATCAACTTTGAAGACCCAATAAACACATCTACTTCAGTTCATTGGTACTTACCCTGTA
TAACCTGAAAATCTTTAAACACCAA
   
Sequence Name 08-K102177-0022-017-C05C-EP09
GenBank Accession LN489680 [GenBank]
Sequencing primer gene specific primer, EP09: 5'-GTTGGTGTTGAAAGATTTTCAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K102177-0022-017-C05C-EP09
CTGAGTAATGTGTTATTGGGTCTTCACAGTTGATATTAAGCTTATTATATAGTCTTCTCT
CTAAATCCAACTCATGGTGGCTCCATTCAGGTATGGGCCTTCGTTCCAATATGCTGGTGA
TTACACAGACAACAAGGACTTAGACATTTTCAGGAGGCGAAAAACAAGAGTCATAGCTAT
AGATGCCATGCCTGACCCAGGAATGGGACAGTACAAACTTGATGCCCTCATTCGGTAATC
AAAACTTGTCTTTCAGCCAGTTTTTCATTCTTTCCTTTTTTTTATTTTGTTTGTGAATTA
CTCAATTGCTCCTTTTGTTTCTGTAGAGAGGTTAATAAGGCTTTTAGCGGATACATGCAT
CAATGTAAATATAATATCGATGTTAAGCATGATCCTGAGGCATCATCTTCGGTAAGACCA
CAATTATGCTCCATACATGAATTTAGCATACATGTTAAGCATTTTGTTTTTCTTGACTAG
ACCAAGATTAAATTTACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATACCT

Last Updated on 06.12.2018 16:11