Sequences from confirmation amplicon


Confirmation sequences for line 017G03 and hit At4g02510 are shown.
Line ID 017G03
Locus At4g02510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1104974
Primers used for PCR MC30 - 8474, show primer details
   
Sequence Name 85-K035351-022-017-G03C-MC30
GenBank Accession LN514501 [GenBank]
Sequencing primer gene specific primer, MC30: 5'-CAGTTTAAAGCGTAGCACAAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K035351-022-017-G03C-MC30
CATAGAAGCGAGGGTAATTCGGTAATTTCATCATCATCCTCAAAACACAAACTGAGAAGA
TAAAATCCCAAGGCAAAGAAGAAAAACGAAGAGTCATTTTTTGTTCGCCGAGTGACCTTA
TCTCTCCCAATTCTCTCTTCTTCAAAAGAGAGAGAGCAGCCAAATTCTCAATCATAAAAA
CCTTCACACAAAAAAGGGTAAATTCCTTTAAACCCTATCTATCTATCGCTATCTCCTTCC
TCCACAAGTTTGGCGGAGAAAGAGTCTCAGTAGCAAAGCGGAAATGGACTCAAAGTCGGT
TACTCCAGAACCAACCAACCCCTTCTACGCTTCTTCGGGGCAATCAGGTTTTTCTTCTTT
CTCGTTTATATCTTCTTTAATCTGCGTTTTTGGGAATAGGGTTTTAATCGGAAGGATATT
TTGTTATCAGGAAAAACCTATGCTTCTGTTGTCGCCGCCGCTGCTGCTGCAGCCGCCGAT
AAGGAGGATGGTCAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTAGAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTCA
   
Sequence Name 37-K035351-022-017-G03C-8409
GenBank Accession LN502527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K035351-022-017-G03C-8409
GAGCCTATTGACCATCCTCCTTATCGGCGGCTGCAGCAGCAGCGGCGGCGACAACAGAAG
CATAGGTTTTTCCTGATAACAAAATATCCTTCCGATTAAAACCCTATTCCCAAAAACGCA
GATTAAAGAAGATATAAACGAGAAAGAAGAAAAACCTGATTGCCCCGAAGAAGCGTAGAA
GGGGTTGGTTGGTTCTGGAGTAACCGACTTTGAGTCCATTTCCGCTTTGCTACTGAGACT
CTTTCTCCGCCAAACTTGTGGAGGAAGGAGATAGCGATAGATAGATAGGGTTTAAAGGAA
TTTACCCTTTTTTGTGTGAAGGTTTTTATGATTGAGAATTTGGCTGCTCTCTCTCTTTTG
AAGAAGAGAGAATTGGGAGAGATAAGGTCACTCGGCGAACAAAAAATGACTCTTCGTTTT
TCTTCTTTGCCTTGGGATTTTATCTTCTCAGTTTGTGTTTTGAGGATGATGATGAAATTA
CCGAATTACCCTCGCTTCTATGTTGGTGAAGTTTCCAAGAGGGCTCTCTTTTGTGCTACC
TNTTAAACTG

Last Updated on 06.12.2018 16:11