Sequences from confirmation amplicon


Confirmation sequences for line 017G04 and hit F25P22 are shown.
Line ID 017G04
Locus F25P22 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27759587
Primers used for PCR MJ21 - 8474, show primer details
   
Sequence Name 01-K023622-022-017-G04C-8409
GenBank Accession LN486409 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K023622-022-017-G04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTATTATCGTACATTGAT
TGCATTTGCTATATGGATTTAAGATTTCATTGGTTATACAGTTTTAGTTATTTTACCGTT
TAGTATTAAACACTTTGGTAAGAAGAATAAAAAGTTGTATGGATTATATTCAAGAATAGT
TTGATTGATGATATTCACTGGTTTCAAATAGTATTAACATTTTTAGTTAAATAGTACCTA
TTTATGTTAAACAAAAGAAATATTTTTGTATGATACTTATAATGTGATGTGATAGTATAC
GAGCTAAATTAATGTTGGCTATTTATAATTTTTTTTTTTTTTGGAAAACAATA
   
Sequence Name 02-K023622-022-017-G04C-MJ21
GenBank Accession LN486864 [GenBank]
Sequencing primer gene specific primer, MJ21: 5'-GCATAATCCACATATTGTTTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K023622-022-017-G04C-MJ21
AAAAAAAAAATTATAAATGGCCAACATTAATTTAGCTCGTATACTATCCCATCCCATTAT
AAGTATCATACAAAAATATTTCTTTTGTTTAACATAAATAGGTACTATTTAACTAAAAAT
GTTAATACTATTTGAAACCAGGGAATATCATCAATCAAACTATTCTTGAATATAATCCAT
ACAACTTTTTATTCTTCTTACCAAAGGGTTTAATACTAAACGGTAAAATAACTAAAACTG
TTTAACCAATGAAATCTTAAATCCATATAGCAAATGCAATCAAGGTACGATAATAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTTTTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATGGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11