Sequences from confirmation amplicon


Confirmation sequences for line 018C06 and hit At5g03140 are shown.
Line ID 018C06
Locus At5g03140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:738556
Primers used for PCR KE46 - 8474, show primer details
   
Sequence Name 16-K100994-0022-018-C06C-8409
GenBank Accession LN493364 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K100994-0022-018-C06C-8409
CCATTTACATTGAATATATCCTGTTAATTTATGATCTAATGCCTAATGGATCACTTGATA
AAGCACTCTACGAGTCTCCGACTACATTACCCTGGCCTCACCGGAGAAAAATACTTCTTG
GAGTCGCTTCTGCTTTGGCTTATTTGCATCAAGAGTGCGAGAATCAGATCATTCACCGTG
ATGTTAAAACAAGTAACATTATGCTCGACGCGAATTTTAATCCGAAGTTAGGTGATTTTG
GACTCGCGAGGCAAACGGAGCACGATAAATCGCCGGACGCAACCGCCGCCGCTGGAACAA
TGGGGTATTTAGCGCCGGAGTATTTGTTAACCGGTCGAGCGACGGAGAAAACCGACGTTT
TTAGCTACGGAGCCGTTGTTTTAGAAGTATGCACTGGTAGAAGACCCATAACCCGACCCG
AACCGGAACCCGGTTTAAGACCCGGTTTGAGGTCGAGTTTGGTAGATTGGGTTTGGGGTT
TGTATAGAGAAGGGAAGTTATTGACCGCCGTGGACGAGCGGTTGAGTGAGTTTAACCCGG
AGGAGATGAGTCGGGTTATGATGGTGGGCCTTGCTTGTTCACAACCCGATCCGGTCACCA
GGCCCACTATGAGAAGTGTGGTTCAGA
   
Sequence Name 16-K100998-0022-018-C06C-KE46
GenBank Accession LN493365 [GenBank]
Sequencing primer gene specific primer, KE46: 5'-TCTGAACCACACTTCTCATAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K100998-0022-018-C06C-KE46
AGCAGGCCCACCATCATAACCCGACTCATCTCCTCCGGGTTAAACTCACTCAACCGCTCG
TCCACGGCGGTCAATAACTTCCCTTCTCTATACAAACCCCAAACCCAATCTACCAAACTC
GACCTCAAACCGGGTCTTAAACCGGGTTCCGGTTCGGGTCGGGTTATGGGTCTTCTACCA
GTGCATACTTCTAAAACAACGGCTCCGTAGCTAAAAACGTCGGTTTTCTCCGTCGCTCGA
CCGGTTAACAAATACTCCGGCGCTAAATACCCCATTGTTCCAGCGGCGGCGGTTGCGTCC
GGCGATTTATCGTGCTCCGTTTGCCTCGCGAGTCCAAAATCACCTAACTTCGGATTAAAA
TTCGCGTCGAGCATAATGTTACTTGTTTTAACATCACGGTGAATGATCTGATTCTCGCAC
TCTTGATGCAAATAAGCCAAAGCAGAAGCGACTCCAAGAAGTATTTTTCTCCGGTGAGGC
CAGGGTAATGTAGTCGGAGACTCGTAGAGTGCTTTATCAAGTGATCCATTAGGCATTAGA
TCATAAATTAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGG
ATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
TCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11