Sequences from confirmation amplicon


Confirmation sequences for line 021C04 and hit At1g77300 are shown.
Line ID 021C04
Locus At1g77300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29041071
Primers used for PCR PP12 - 8474, show primer details
   
Sequence Name 06-K102723-0022-021-C04C-8409
GenBank Accession LN488761 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K102723-0022-021-C04C-8409
CGGACTGAGCCATTTTGGCTTTAATTTCCAATTATTTTCATGTGTATGCAGGTTCATAAT
ATTGCGCGGAGCTTCCGAGACAGATGGATACCTAAGCATTTTAGAAAACCTTGGCGCATC
AACAGGGAGGAGAGATCGGAGTCTATGAGGTCACCTATAAACAGAAGGTTCAGAGCATCA
CAAGAACCTCGATATGATCATCAGTCCCCAAGACCTGCAGAACCAGCTGCGTCTGTCACA
TCATCAAAGGCTGCAACTCCTGAAACAGCATCTGTATCTGAGGGATATTCAGAACCTAAT
TCCGGTCTTCCTGAGACAAATGGACGCAAGCGCAAAAGCAGATGGGACCAGCCGTCTAAG
ACAAAAGAACAAAGAATCATGACTATCTTGTCCAAACAAACA
   
Sequence Name 22-K102728-0022-021-C04C-PP12
GenBank Accession LN496067 [GenBank]
Sequencing primer gene specific primer, PP12: 5'-CTGTTTGTTGGGACAAGATAGTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K102728-0022-021-C04C-PP12
GCTGGTCCCATCTGCTTTTGCGCTTGCGTCCATTTGTCTCAGGAAGACCGGAATTAGGTT
CTGAATATCCCTCAGATACAGATGCTGTTTCAGGAGTTGCAGCCTTTGATGATGTGACAG
ACGCAGCTGGTTCTGCAGGTCTTGGGGACTGATGATCATATCGAGGTTCTTGTGATGCTC
TGAACCTTCTGTTTATAGGTGACCTCATAGACTCCGATCTCTCCTCCCTGTTGATGCGCC
AAGGTTTTCTAAAATGCTTAGGTATCCATCTGTCTCGGAAGCTCCGCGCAATATTATGAA
CCTGCATACACATGAAAATAATTGGAAATTAAAAGCCAAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11