Sequences from confirmation amplicon


Confirmation sequences for line 024B09 and hit At1g75430 are shown.
Line ID 024B09
Locus At1g75430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28309226
Primers used for PCR JU12 - 8474, show primer details
   
Sequence Name 49-K020890-022-024-B09C-8409
GenBank Accession LN506690 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K020890-022-024-B09C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGCCATGAATATAGCCAGAAT
CAATTGCTAATCTTCCAAACAATTATGAGAAATAGTCTAAATTTTATATCAAATTTAAAA
GTAGTCTAGCTTTTTTATTAATTAACAAAATAGTCAAATTTTAAACCTTAAAGCTTAACA
ACTATCTAAAGTATAAAAACTAAAAATTAAGTCCAAATTTTAAAACCCTAACTCATTAAA
CCCAAATGTTTTGATCATAAAATCTAAACAATATGGTTAATTTATTTGATACTTTGATTT
TTTGAAGGGTTTTCCTCCTAATTATGCAATATGTTTT
   
Sequence Name 50-K020890-022-024-B09C-JU12
GenBank Accession LN507034 [GenBank]
Sequencing primer gene specific primer, JU12: 5'-AAACCAAAGGAAAACATATTGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K020890-022-024-B09C-JU12
GAAAACCCTTCAAAAAATCAAAGTATCAAATAAATTAACCATATTGTTTAGATTTTATGA
TCAAAACATTTGGGTTTAATGAGTTAGGGTTTTAAAATTTGGACTTAATTTTTAGTTTTT
ATACTTTAGATAGTTGTTAAGCTTTAAGGTTTAAAATTTGACTATTTTGTTAATTAATAA
AAAAGCTAGACTACTTTTAAATTTGATATAAAATTTAGACTATTTCTCATAATTGTTTGG
AAGATTAGCAATTGATTCTGGCTATATTCATGGCTATATTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
Primers used for PCR FL42 - 3144, show primer details
   
Sequence Name 96-K028754-022-024-B09C-3144
GenBank Accession LN515364 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K028754-022-024-B09C-3144
GGGTACCCGGGGATCAGATTGTCGTTTTCCGCCTTCGGTTTAAACTATATTCATCATCGT
TTGAATGGTGTCTGGGATCGTTGCAACAAACTGAGAAGCTTTGTGATTTTGATATGAATC
TCATGATTCTCAGGCAAAGACATGAAACCAGAACTGCAAAGTTCGGATTTGATTTCGGAA
GACAAAGCAAAACCGGGTCTTCTCCTTCCTGGAAACAGCTGATTGATTAGAATGTTGTTG
CAAAGCTCAACCTCTCTACCACCTATATCGATAACTTCTTCTACAAGGCATTGCACTGCC
TTAGCATATCTTGAATCCAACAAAGTACCACGGAGAGATGAACACTCATGTCTTACCCTA
AAATCCTCCATCTTCTTCTACTTGTTGAAGAGGAAAAATAATGCAAATAAAAAAAAAAAC
TTTTTGGGTTCTTGTTTTGTTACTCTTTTTTGCTCCACATACATAAAATAAGTAACCAAG
AAGCAATCAATATATATTATTCAGATTCATTTTCTAAAAATTTGAAGTTGACTTG
   
Sequence Name 96-K028754-022-024-B09C-FL42
GenBank Accession LN515365 [GenBank]
Sequencing primer gene specific primer, FL42: 5'-GGGTTTCAAAAGCTACAAGTCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>96-K028754-022-024-B09C-FL42
GTAACAAAACAAGAACCCAAAAAGTTTTTTCTTTTATTCGCATTATTTTTCCTCTTCAAC
AAGTAGAAAAAGATGGAGGATTTTAGGGTAAGACATGAGTGTTCATCTCTCCGTGGTACT
TTGTTGGATTCAAGATATGCTAAGGCAGTGCAATGCCTTGTAAAAAAAGTTATCGATATA
GGTGGTAAAGAGGTTGAGCTTTGCAACAACATTCTAATCAATCAGCTGTTTCCAGGAAGG
AGAAAACCCGGTTTTGCTTTGTCTTCCGAAATCAAATCCAAACTTTGCAGTTCTGGTTTC
ATGTCTTTGCCTGAGAATCATGAGATTCATATCAAAATCACAAAGCTTCTCAGTTTGTTG
CAACAATCCCAGACACCATTCAAACAATGATGAATATAGTTTAAACCGAAGGCGGGAAAC
GACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAG
GAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
CATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACAATGCTCCTC
GTGGG

Last Updated on 06.12.2018 16:11