Sequences from confirmation amplicon


Confirmation sequences for line 025D01 and hit At1g66780 are shown.
Line ID 025D01
Locus At1g66780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24909271
Primers used for PCR BM12 - 8474, show primer details
   
Sequence Name 27-K015839-022-025-D01C-8409
GenBank Accession LN498157 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K015839-022-025-D01C-8409
CATGTAGATTTCCCGGACATGAAGCCATTGGTAGAATCTTCTTCTTCTTCCTCTTCTTTG
GGCACCAACGAGAAACCATTTTCCATTGTCTTGATCAAAACTGTTTTTAGAGAAGCGCTA
CAATCAATAAGATTGAATGGATCCAAATGAAAAAAACTTGGGGAACGATAAAGAACTCTT
CGTTGAATTAGACTAAAAAATAGGGAAATATATGCGTTGACCTTGTGAAATGAATTAAAC
AATGACGCGAAGACGTGGTCTCCATAAAAGTTGAGTAGGTAGAGTTATGGGAACTTGTCG
AGGAAGTTAATACAAAACGTTGACTAGATTTTTTTTTTAAACGTTGACTAGTTAGTTGTT
GAATAGATAAAAGAGAATGAAGCTGGTGG
   
Sequence Name 28-K015839-022-025-D01C-BM12
GenBank Accession LN498623 [GenBank]
Sequencing primer gene specific primer, BM12: 5'-TCCACCAGCTTCATTCTCTTTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K015839-022-025-D01C-bm12
AACAACTAACTAGTCAACGTTTAAAAAAAAAATCTAGTCAACGTTTTGTATTAACTTCCT
CGACAAGTTCCCATAACTCTACCTACTCAACTTTTATGGAGACCACGTCTTCGCGTCATT
GTTTAATTCATTTCACAAGGTCAACGCATATATTTCCCTATTTTTTAGTCTAATTCAACG
AAGAGTTCTTTATCGTTCCCCAAGTTTTTTTCATTTGGATCCATTCAATCTTATTGATTG
TAGCGCTTCTCTAAAAACAGTTTTGATCAAGACAATGGAAAATGGTTTCTCGTTGGTGCC
CAAAGAAGAGGAAGAAGAAGAAGATTCTACCAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCA
Primers used for PCR FL44 - 3144, show primer details
   
Sequence Name 08-K028757-022-025-D01C-3144
GenBank Accession LN489598 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K028757-022-025-D01C-3144
GGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCAT
TTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAA
ACTATCAGTGTTTGATGAGAAATCTGAAGATCAGACGAGTTATTATTTGTCAACAGAGAT
GATGAAGAAGGTAAGCTTCATGGCTGCACCAATGGTGGCTGTGGCTGCTTCTCAATACCT
CCTTCAAGTTATCTCCATTGTTATGGCTGGTCACTTGGACGAGCTTTCTCTCTCAGCCGT
TGCTATTGCCACTTCTCTTACCAATGTCACCGGCTTCAGCCTCATCGTAAATTCCTCCTC
CTCCTCCTCCTCCTTCTCTCTCGACTTATAGACCTGTCCAAAGGACAAACATGCTAAGCA
AAAAATTAGGGTCGCCAAATTTTTTTTATTTTTAAATATTAATGCAACTTCACTTTTTTT
AGTAGTTATATCTTTAGGACTACCAATTTTTTGTTTTGATAGAAACTTACACCTTTTCAA
AGTTATGGGAGTGTACACATTTCTTGTTTCATAACTAGGACCACTATTTTTATTGGACCG
ACTTTGCTGACTTAGATCGTTAGTTTGGATTGGCAGGTGCGTTAGAGACACTGTGTGGTC
AAGCTTTTGGAGCTGGGCAATTTAGGAATATCANTGCATACACTTA
   
Sequence Name 09-K028757-022-025-D01C-FL44
GenBank Accession LN490042 [GenBank]
Sequencing primer gene specific primer, FL44: 5'-AAGACATAGCATTGAGCCGTAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K028757-022-025-D01C-FL44
ATGCACTGATATTCCTAAATTGCCCAGCTCCAAAAGCTTGACCACACAGTGTCTCTAACG
CACCTGCCAATCCAAACTAACGATCTAAGTCAGCAAAGTCGGTCCAATAAAAATAGTGGT
CCTAGTTATGAAACAAGAAATGTGTACACTCCCATAACTTTGAAAAGGTGTAAGTTTCTA
TCAAAACAAAAAATTGGTAGTCCTAAAGATATAACTACTAAAAAAAGTGAAGTTGCATTA
ATATTTAAAAATAAAAAAAATTTGGCGACCCTAATTTTTTGCTTAGCATGTTTGTCCTTT
GGACAGGTCTATAAGTCGAGAGAGAAGGAGGAGGAGGAGGAGGAGGAATTTACGATGAGG
CTGAAGCCGGTGACATTGGTAAGAGAAGTGGCAATAGCAACGGCTGAGAGAGAAAGCTCG
TCCAAGTGACCAGCCATAACAATGGAGATAACTTGAAGGAGGTATTGAGAAGCAGCCACA
GCCACCATTGGTGCAGCCATGAAGCTTACCTTCTTCATCATCTCTGTTGACAAATAATAA
CTCGTCTGATCTTCAGATTTCTCATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACG
ACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGG
AAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCAC
ATCAATCCACTTGCT

Last Updated on 06.12.2018 16:11