Sequences from confirmation amplicon


Confirmation sequences for line 025D01 and hit At2g05890 are shown.
Line ID 025D01
Locus At2g05890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:2254813
Primers used for PCR HM51 - 8474, show primer details
   
Sequence Name 09-K032413-022-025-D01C-8409
GenBank Accession LN490063 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K032413-022-025-D01C-8409
ATACAACTATCTAAGAATATAAAAATGTAATAAAAATGTTAGTATGATAAATAAATTAAT
GAATAGTACATCAGAAGATGTGAGGACTTCCTTGATTGTTTGCCTTGGATAATCACCATC
GTCATCCCCAGTTCTTGGTGCATAGAGCCTCTTGTGAAAAGATCCAGAACTTCCCAAAGT
TAACCCATCAGTTGGTATGCTATATATCAAGAAAATATAGTTTGCTTAATATTATGTATA
AAAAAATATATTTAGTGACAAATAAATACAATTACCTTTGCTTAAAAAGATCCAACTCAG
CCAACGTGGGATCCACGAAGACTTGTGAACCATCAAAA
   
Sequence Name 09-K032413-022-025-D01C-HM51
GenBank Accession LN490064 [GenBank]
Sequencing primer gene specific primer, HM51: 5'-TTTTTGATGTTTCACAAGTCTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K032413-022-025-D01C-HM51
CGATGGACTTTTAGCAAGGTAATTGTATTTATTTGTCACTAAATATATTTTTTTATACAT
AATATTAAGCAAACTATATTTTCTTGATATATAGCATACCAACTGATGGGTTAACTTTGG
GAAGTTCTGGATCTTTTCACAAGAGGCTCTATGCACCAAGAACTGGGGATGACGATGGTG
ATTATCCAAGGCAAACAATCAAGGAAGTCCTCACATCTTCTGATGTACTATTCATTAATT
TATTTATCATACTAACATTTTTATTACATTTTTATATTCTTAGATTAGTTTGTTAATTCC
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACAT
TTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTA
TTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11