Sequences from confirmation amplicon


Confirmation sequences for line 030C06 and hit At5g16820 are shown.
Line ID 030C06
Locus At5g16820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5532123
Primers used for PCR RE34 - 8474, show primer details
   
Sequence Name 13-K025944-022-030-C06C-8409
GenBank Accession LN491876 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K025944-022-030-C06C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGTAACTTGCTTCGGGT
ACTTGATTCGTTGCTGACTGAACTGTGTTGGGTGAAAACTCGGCCAATGTTACTCCAGAA
ACTCTACTTGAAGGGTTCCCATTGTCTACAGAGGTAGAACTGGGAACATCACCCAATAGG
AAACTGTCAGGATTGTTTGAAACTGATTCATACCGAGGTGAGGTACTAGTATTTAAGAAC
TGTCGAAGCATATTTTGTGCTGCTTCGTTTATCGACGGCTGATATCTAACAATCTGGCGG
TTAAGACCATTAGCCACATTGTCACCACGATTCTCCTGCTCATCTACAGGAAGTCTCCTC
TTTTTGTTGCTTCCTGGAATTTGTCTGTTGCCATCATTATTATTCTGTTGTACTAACTGG
TTTAAGAAACCTGGACTTTGAACAGCCTTTGCTAAAAACGAC
   
Sequence Name 14-K025944-022-030-C06C-RE34
GenBank Accession LN492346 [GenBank]
Sequencing primer gene specific primer, RE34: 5'-AACAAATGATGTCGTTTTTAGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K025944-022-030-C06C-RE34
GCTGTTCAAAGTCCAGGTTTCTTAAACCAGTTAGTACAACAGAATAATAATGATGGCAAC
AGACAAATTCCAGGAAGCAACAAAAAGAGGAGACTTCCTGTAGATGAGCAGGAGAATCGT
GGTGACAATGTGGCTAATGGTCTTAACCGCCAGATTGTTAGATATCAGCCGTCGATAAAC
GAAGCAGCACAAAATATGCTTCGACAGTTCTTAAATACTAGTACCTCACCTCGGTATGAA
TCAGTTTCAAACAATCCTGACAGTTTCCTATTGGGTGATGTTCCCAGTTCTACCTCTGTA
GACAATGGGAACCCTTCAAGTAGAGTTTCTGGAGTAACATTGGCCGAGTTTTCACCCAAC
ACAGTTCAGTCAGCAACGAATCAAGTACCCGAAGCAAGTTACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACTTTTTGATAAAACGACAAATTACGATCCGTCGTATTTATGG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
Primers used for PCR FL53 - 3144, show primer details
   
Sequence Name 90-K028754-022-030-C06C-3144
GenBank Accession LN514994 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K028754-022-030-C06C-3144
GGGTAACCCGGGGATTCAGAATTGTCGTTTTCCCGCCTTCCGGTTTAAACTATCAGTGTT
TGCACCAGCAGACTCTTGGAGCCCTGAATTTTGATTTAGTTCGGATCGCGAGACAGATAG
TGGAGAGTGTTTTGATCCAATAATGGCTGTTTTAGATGAGTCAGAAGGCGATGCAATTTC
TCCTGAAGGTGAGGGCAAGATGAATGAGTTACTGGAGGGAGTCCCTAAGCTGCCCGGAAT
CCAAGATCCATTCTGGGAACAGTTCTTTTCTGTTGAACTCCCAGCGATTGCAGATACAGA
CGATATTCTATCAGGATCAGTGGAGAATAATGACTTGGTATTGGAACAAGAACCAAACGA
GTGGACCCGTAATGAACAACAAATGAAGTATCTTACTGAACAAATGGGACTGCTTTCCTC
AGAAGCACAGAGGAAATAAAGGTAAGAACATCGTTAAGTTCAAACATGTTTCTCTGCATT
GTTGTATATCTTGAGAGTTATCAATTGTCTCTCACAATGTAGATTTTCAGGGGAGGTTGC
AA
   
Sequence Name 90-K028754-022-030-C06C-FL53
GenBank Accession LN514995 [GenBank]
Sequencing primer gene specific primer, FL53: 5'-CTTCATATCTCCTTTTGCAACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K028754-022-030-C06C-FL53
ACTCTCAAGATATACAACAATGCAGAGAAACATGTTTGAACTTAACGATGTTCTTACCTT
TATTTCCTCTGTGCTTCTGAGGAAAGCAGTCCCATTTGTTCAGTAAGATACTTCATTTGT
TGTTCATTACGGGTCCACTCGTTTGGTTCTTGTTCCAATACCAAGTCATTATTCTCCACT
GATCCTGATAGAATATCGTCTGTATCTGCAATCGCTGGGAGTTCAACAGAAAAGAACTGT
TCCCAGAATGGATCTTGGATTCCGGGCAGCTTAGGGACTCCCTCCAGTAACTCATTCATC
TTGCCCTCACCTTCAGGAGAAATTGCATCGCCTTCTGACTCATCTAAAACAGCCATTATT
GGATCAAAACACTCTCCACTATCTGTCTCGCATCCAACTAAATCAAATTCAGGGCTCCAA
GAGTCTGCAGGTGCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCC
CCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTT
GCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGG

Last Updated on 06.12.2018 16:11