Sequences from confirmation amplicon


Confirmation sequences for line 031D04 and hit At4g37730 are shown.
Line ID 031D04
Locus At4g37730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:17723984
Primers used for PCR DR10 - 8474, show primer details
   
Sequence Name 51-K018975-022-031-D04C-DR10
GenBank Accession LN507354 [GenBank]
Sequencing primer gene specific primer, DR10: 5'-GTGACTTTGTTTACGCATTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K018975-022-031-D04C-DR10
CCGGTTTGACTCCATCCTCTTCCTCTTCCGCTCGTCGGTCATCTCCGGTTGGATCATTTT
TTTCCGGTTATGGTTATTCTCTCCCGAACCGAAATTTGTGATGGCTGTCAATTGTTTTGT
GTCGTCCGAGTCCGGTTCATCTGCTTGAGAGCACGGTAGATCGTTTGATATGTCGGCGTC
GTCACAACGAGGATTAATTTTGACGAAACCGGCACAAGATTCGTCAGAACCGGTTTTCAT
GTCATGAGAACCGGAATTAATTTTGACGGAACCAGTGTCGGATTCACCATAACAAGGACT
AGGGATGGCCGGTTCAAGAGACATTTGGTCCACGAAAAAGAGATCAGAGCAATCCCATTC
CCAAGGAGTGAACCCGGTTTGGAAACCCGAGAATTGATTAACCAAACCCGGTTCGGAGAA
TGAAAAGGCCGGTGAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCT
   
Sequence Name 55-K018975-022-031-D04C-8409
GenBank Accession LN508664 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K018975-022-031-D04C-8409
CATGTAGATTCACCGGACCTTTTCATTCTCCGAACCGGGTTTGGTTAATCAATTCTCGGG
TTTCCAAACCGGGTTCACTCCTTGGGAATGGGATTGCTCTGATCTCTTTTTCGTGGACCA
AATGTCTCTTGAACCGGCCATCCCTAGTCCTTGTTATGGTGAATCCGACACTGGTTCCGT
CAAAATTAATTCCGGTTCTCATGACATGAAAACCGGTTCTGACGAATCTTGTGCCGGTTT
CGTCAAAATTAATCCTCGTTGTGACGACGCCGACATATCAAACGATCTACCGTGCTCTCA
AGCAAATGAACCGGACTCGGACGACACAAAACAATTGACAGCCATCACAAATTTCGGTTC
GGGAGAGAATAACCATAACCGGAAAAAAATGATCCAACCGGAGATGACCGACAAGCGGAA
AAGGAAAAGGATGGAGTCAAACCGGGAATCAGCGAAACGGTCAAGAATGCGT
Primers used for PCR FL56 - 3144, show primer details
   
Sequence Name 80-K028756-022-031-D04C-3144
GenBank Accession LN513890 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K028756-022-031-D04C-3144
CTCTATATAAGGAAGTTCATTTCATTTGCAGAGGACAGGGTACCCGGGGATCAGATTGTC
GTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAAGGGAAAAAGGGAAGAATTGCATTG
TTGGAGCTTGATATTTATGGACAAACCAAGGGGTAATTTGGTCATATTTCCAAAATACGA
TGTAAGACCGTAGACTATACTGTATAAACAGCTTTTGAGTCATGTTGCACTATGGTGGGG
TTAAAAACTTGAAAATTTTTATTTGGCAGAGCCAACTTTTGTAAAAGCAGGAAATATTGT
GTAGGGCACCAATAGCTTGTTTTGGCGTCCA
   
Sequence Name 81-K028756-022-031-D04C-FL56
GenBank Accession LN514008 [GenBank]
Sequencing primer gene specific primer, FL56: 5'-ATATGGACCCAAAACAAGCTATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>81-K028756-022-031-D04C-FL56
AAAGTTGGCTCTGCCAAATAAAAANTTTTCAAGTTTTTAACCCCACCATATTGCAACATG
ACTCAAAAGCTGTTTATACAGTATAGTCTACGGTCTTACATCGTATTTTGGAAATATGAC
CAAATTACCCCTTGGTTTGTCCATAAATATCAAGCTCCAACAATGCAATTCTTCCCTTTT
TCCCTTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTA
CCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATA
GTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGA
AGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGGTCCATCTTT

Last Updated on 06.12.2018 16:11