Sequences from confirmation amplicon


Confirmation sequences for line 033D05 and hit At3g54350 are shown.
Line ID 033D05
Locus At3g54350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20124914
Primers used for PCR DC77 - 8474, show primer details
   
Sequence Name 68-K032737-022-033-D05C-DC77
GenBank Accession LN511937 [GenBank]
Sequencing primer gene specific primer, DC77: 5'-TTGAATAACCATGTCAGCTTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K032737-022-033-D05C-DC77
CTTAGCAGTGTTATTGCGGTATAGTATAGTATTATGGGAAACAAGTTACACAATCTTGAT
GTGGCAAGGTATAATTGTTGCTCTAGAACAAATTATACCATTTCGCGAGGAACATAATAT
ATTGCTGTGTGATCACTGATGATCTGTATGTATCATGCTTCTCTCCACGGGGAAAAGACT
GTCAAATATTTTTTCTTAAATTTGGTTTTCTGTTCAGTTATGAGAACATAATATGATTGA
AAAAATATCCTTTTGTCTTTTTACAGATGGCTCATCAATCACCACAAGATAGATAATTAT
GTTCATTGCTTCTCTCATATAGCATTTTATATATCAAGCTAGGCTAGCCAAAAAGTTTCT
CTACTAAATTAGTGCTTCTGTATGTATGTATTGCGTGTTTCTTCTTTCAGCTTGTCTGGT
AATCTTGCAATTAAGTGAACCAGAATCCTTAATTTATTTGCAGGCTGGTGCTTCTTTGGA
ATCGCTTGCTAAAGGTGCTGTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 36-K032737-022-033-D05C-8409
GenBank Accession LN502117 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K032737-022-033-D05C-8409
CGGAATGAGCCTTTACAATTGAATATATCCTGCACAGCACCTTTAGCAAGCGATTCCAAA
GAAGCACCAGCCTGCAAATAAATTAAGGATTCTGGTTCACTTAATTGCAAGATTACCAGA
CAAGCTGAAAGAAGAAACACGCAATACATACATACAGAAGCACTAATTTAGTAGAGAAAC
TTTTTGGCTAGCCTAGCTTGATATATAAAATGCTATATGAGAGAAGCAATGAACATAATT
ATCTATCTTGTGGTGATTGATGAGCCATCTGTAAAAAGACAAAAGGATATTTTTTCAATC
ATATTATGTTCTCATAACTGAACAGAAAACCAAATTTAAGAAAAAATATTTGACAGTCTT
TTCCCCGTGGAGAGAAGCATGATACATACAGATCATCAGTGATCACACAGCAATATATTA
TGTTCCTCGCGAAATGGTATAATTTGTTCTAGAGCAACAATTATACCTTGCCACATCAAG
ATTGTGTAACTTGTTTCCCATAATACTATACTATACCGCATATAACACTGGCTTAAGATG
TTAACAGGATCAAACAAAAAGCTGA

Last Updated on 06.12.2018 16:11