Sequences from confirmation amplicon


Confirmation sequences for line 034C03 and hit At1g78290 are shown.
Line ID 034C03
Locus At1g78290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29458813
Primers used for PCR BG46 - 8474, show primer details
   
Sequence Name 78-K101672-0022-034-C03C-8409
GenBank Accession LN513661 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K101672-0022-034-C03C-8409
CCATTTACATTGAAGTTCATCGAGCGAGGCCAAAAGGTCTCTAACTCTCTTGCACCTAAT
TCAAGAAATTTTTGTTTCTATCACTTTTGTCTGATTTTGTTTATTTGTTGATGTTTATGA
AGATTGATGAACATGTACAAAGAGAAATCATGAACCATAGGTCGCTGATCCATCCCAATA
TAATAAGATTCAAGGAGGTATAATACAACAATGTCTTCTCTTTTGATGTTACTTTATCTA
TCAAATTGATAATTGTGTGAAAACATAGGTTTTATTGACGGCAACACATTCTGGCGTTAG
TAATGGAATACGTGAAAAACCAA
   
Sequence Name 78-K101679-0022-034-C03C-BG46
GenBank Accession LN513662 [GenBank]
Sequencing primer gene specific primer, BG46: 5'-CGTATTCCATTACTAACGCCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K101679-0022-034-C03C-BG46
CTATGTTTTCACACATTATCAATTTGATAGATAAAGTAACATCAAAAGAGAAGACATTGT
TGTATTATACCTCCTTGAATCTTATTATATTGGGATGGATCAGCGACCTATGGTTCATGA
TTTCTCTTTGTACATGTTCATCAATCTTCATAAACATCAACAAATAAACAAAATCAGACA
AAAGTGATAGAAACAAAAATTTCTTGAATTAGGTGCAAGAGAGTTAGAGACCTTTTGGCC
TCGCTCGATGAACTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCAGCGTAATTATAAT

Last Updated on 06.12.2018 16:11