Sequences from confirmation amplicon


Confirmation sequences for line 034C06 and hit At1g75130 are shown.
Line ID 034C06
Locus At1g75130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28201657
Primers used for PCR MS39 - 8474, show primer details
   
Sequence Name 17-K016841-022-034-C06C-8409
GenBank Accession LN493653 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K016841-022-034-C06C-8409
GTAGATTTCCCGGACATGTAGATTACAATTTCGCGTAGTGTTCATGAAGCCATTCGTGGT
AGTGAGGCGCCACACGGTGGACGAACTCATGAGGATTTCTCCCGGAAGGAATCGGCTTTG
ACTTCGCCTCCGCCGTTAGCCGGCTAACCTCGCCGGAGTTTCCACTGAATATCCGGTAAC
TAGGACCCGTCACGCTCTGCTTCTTGAAGTGAGATTGGATTCTCCACGGAACCCATATAT
TACTGTAGATGAATCTGAAGACAAGGAAGAAAAAGACCAAGACCAAGATGAAGAACACTG
CCATATCTTGACGTGTTAACCTCAAGTCTCTCTCTGGTTCAGTTTCATTTATTTGATCTT
GAATTTTTTTTAATCTGTTCTTAATCTTGAGATTGTGAAAATTACAGTTAAGAAGCATTA
AACTAACAATAAGTGACCGGANAAGAA
   
Sequence Name 18-K016841-022-034-C06C-MS39
GenBank Accession LN494096 [GenBank]
Sequencing primer gene specific primer, MS39: 5'-TTTCTTCTCCGGTCACTTATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016841-022-034-C06C-MS39
ATGCTTCTTAACTGTAATTTTCACAATCTCAAGATTAAGAACAGATTAAAAAAAATTCAA
GATCAAATAAATGAAACTGAACCAGAGAGAGACTTGAGGTTAACACGTCAAGATATGGCA
GTGTTCTTCATCTTGGTCTTGGTCTTTTTCTTCCTTGTCTTCAGATTCATCTACAGTAAT
ATATGGGTTCCGTGGAGAATCCAATCTCACTTCAAGAAGCAGAGCGTGACGGGTCCTAGT
TACCGGATATTCAGTGGAAACTCCGGCGAGGTTAGCCGGCTAACGGCGGAGGCGAAGTCA
AAGCCGATTCCTTCCGGGAGAAATCCTCATGAGTTCGTCCACCGTGTGGCGCCTCACTAC
CACGAATGGCTTCATGAACACTACGCGAAATTGTAATCTACATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTNTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11