Sequences from confirmation amplicon


Confirmation sequences for line 035F11 and hit At1g32090 are shown.
Line ID 035F11
Locus At1g32090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11540110
Primers used for PCR RU04 - 8474, show primer details
   
Sequence Name 22-K023371w-022-035-F11C-RU04
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, RU04: 5'-AAGAGACACCAGAGGTAAGAGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K023371w-022-035-F11C-RU04
AAACATGAAACAAGTCGTCTAGTCCTGTGACAGAGCTCGGTACTTCTTCCCATCACCATC
ATGTCTACAATTCCACTTCCCCTTCTTCTCACTACGCTTCAGCTTATGAGCAGTCATCGT
CTCAGTATGAATATCACTACAATACTCATCAGTACGAGGAGCATGAGTACCGTTACAATT
GATCTCTAGACAGAAGAAAAATATGTAATCTCTTATGAGTGTCATAACTAAAGGTTTCTT
TTGGTCTAGGTTTAGGTTATTTGTTAGCTGACTTTGGTTTCGGTTTTAAAGGTTAGGAAA
TTGGTGTTAGTGTCATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCTACT
   
Sequence Name 18-K023371w-022-035-F11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K023371w-022-035-F11C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATGACACTAACACCAATTTCCTA
ACCTTTAAAACCGAAACCAAAGTCAGCTAACAAATAACCTAAACCTAGACCAAAAGAAAC
CTTTAGTTATGACACTCATAAGAGATTACATATTTTTCTTCTGTCTAGAGATCAATTGTA
ACGGTACTCATGCTCCTCGTACTGATGAGTATTGTAGTGATATTCATACTGAGACGATGA
CTGCTCATAAGCTGAAGCGTAGTGAGAAGAAGGGGAAGTGGAATTGTAGACATGATGGTG
ATGGGAAGAAGTACCGAGCTCTGTCACAGGACTAGACGACTGTGTTTCATGTTTGTCCAC
TCTTACCTCTG

Last Updated on 06.12.2018 16:11