Sequences from confirmation amplicon


Confirmation sequences for line 036D10 and hit At2g31310 are shown.
Line ID 036D10
Locus At2g31310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13353469
Primers used for PCR DC79 - 8474, show primer details
   
Sequence Name 58-K032606-022-036-D10C-DC79
GenBank Accession LN509615 [GenBank]
Sequencing primer gene specific primer, DC79: 5'-GTGTTTGGAGCTAGCAACTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032606-022-036-D10C-DC79
TGACCGATGTGACGCCGTACGAACCATCTCTTACGAGGCTCACTCTCGTCTCCATGATCC
TATTTACGGCTGCGTCTCCCAAATCTTCTCCCTCCAACAACAGGTATTATACTTTTTCTT
CGGAAATGTATACATAATTTTTTTTTGCTTTTGCTTCATCATCTCTAGTGTACTTACAAG
TAACTTCACTGACTTTGTTCTTAAGGTTGTTAGTTTACAAGCACAAGTGGTTCTACTTAG
AGAAGAAGCTTCGAGAAGGTTCCCTCAAGATGATCCTGGTTGTAATATGAAGCAACAATT
GTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTC
   
Sequence Name 58-K032606-022-036-D10C-8409
GenBank Accession LN509614 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032606-022-036-D10C-8409
CCTTAAATTGAAATATTCACAATTGTTGCTTCATATTACAACCAGGATCATCTTGAGGGA
ACCTTCTCGAAGCTTCTTCTCTAAGTAGAACCACTTGTGCTTGTAAACTAACAACCTTAA
GAACAAAGTCAGTGAAGTTACTTGTAAGTACACTAGAGATGATGAAGCAAAAGCAAAAAA
AAATTATGTATACATTTCCGAAGAAAAAGTATAATACCTGTTGTTGGAGGGAGAAGATTT
GGGAGACGCAGCCGTAAATAGGATCATGGAGACGAGAGTGAGCCTCGTAAGAGATGGTTC
GTACGGCGTCACATCGGTCATGATCAGGGAGATGAGAAATGAGCTTAGAGAAGTTGCT
Primers used for PCR FP23 - 8474, show primer details
   
Sequence Name 84-K035342-022-036-D10C-8409
GenBank Accession LN514383 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K035342-022-036-D10C-8409
GGAATGAGCCATTTACAATTGAATATATTTACATGAAGCCATGTAGATTGCTCAACAAAT
GCCACAAGATCTTCACAATTGGTTTCACCAAGGAATCTTGGACTCAGACTTCGACCCAAT
GAGTGGTACTACACGCGACAATGAGCGGTCCATGGATCATAATGAGTCTCCTTGTAGCTC
AAATGAATCACTTAATTACTACCAGGAGGTCAATTTTCCTTGGTCTGTTTGAGTACAAGT
GACTACTTTGTCAGAGATGGAACCTTACAATCTTTCTATTGTATCGACTTTTCGACATTT
TTCATAGTAGAGATCGAATCATGCAACCATTTTCTCTTTGAGAATAACCACTTCTTCGAT
GATCTTTCATAGGTACAACATCAGATCTGAACAAGTGTCAAGTCTTAAGAAGTGTCATTA
CTGAGAAAAAGCATAACAAAATGTAGTGTACGCTGTTTTAAAACCAGAAACATC
   
Sequence Name 84-K035342-022-036-D10C-FP23
GenBank Accession LN514384 [GenBank]
Sequencing primer gene specific primer, FP23: 5'-ACAGATTTAACTCTCTGGCGTTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K035342-022-036-D10C-FP23
CGTAACTACATTTTGTTATGCTTTTTCTCAGTAATGACACTTCTTAAGACTTGACACTTG
TTCAGATCTGATGTTGTACCTATGAAAGATCATCGAAGAAGTGGTTATTCTCAAAGAGAA
AATGGTTGCATGATTCGATCTCTACTATGAAAAATGTCGAAAAGTCGATACAATAGAAAG
ATTGTAAGGTTCCATCTCTGACAAAGTAGTCACTTGTACTCAAACAGACCAAGGAAAATT
GACCTCCTGGTAGTAATTAAGTGATTCATTTGAGCTACAAGGAGACTCATTATGATCCAT
GGACCGCTCATTGTCGCGTGTAGTACCACTCATTGGGTCGAAGTCTGAGTCCAAGATTCC
TTGGTGAAACCAATTGTGAAGATCTTGTGGCATTTGTTGAGCAAATCTACATGGCTTCAT
GTAAAATATATTCAATT

Last Updated on 06.12.2018 16:11