Sequences from confirmation amplicon


Confirmation sequences for line 038C07 and hit At2g46400 are shown.
Line ID 038C07
Locus At2g46400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19044434
Primers used for PCR IS25 - 8474, show primer details
   
Sequence Name 49-K017389-022-038-C07C-IS25
GenBank Accession LN506668 [GenBank]
Sequencing primer gene specific primer, IS25: 5'-AAGATCCTTCTCTTTTCCACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017389-022-038-C07C-IS25
GGTCGAAGATTGAAACCAATAATAAATACAAAAATAAAAATTAATACTTATTGTCTAAGC
ACAAGGACGAGTACCATCATAGAATCTCCATCATTCATGTCTATAAATACATCTAGATAC
TCACAGACGAAACTCACATCTCCCACCAATCTCACTCAAAAGAAAATCTAAGAAAAGATT
CTTAATTTTCTGAAGTGAATGCAAAGATGATGATGGAAGAGAAACTTGTGATCAACGAAT
TGGAACTAGGGAAAGAGCTTGCTAACCGATTGATGAACAATCTCAAACACACTTCTTCTG
TCGATTCCAACAAAACCTTGATCTCTGATATCCTCCGTATTTACCAGAATGCCATTTTCA
TGTTGAGCTTCAACCAAGACAAGAACATCCTTAAGCGAAGCCTTGAGATCGATGGAAAAG
ATTCTAAAAACGTCTTCAAAAAGAGGTAAATTGAGCTAAAAGATTGGTTTTTTGTCTCTC
TTTCAGTTCCAAACTGTGCTCATGTGTCTGATTCCTCTGTTTTTTTTTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATA
   
Sequence Name 50-K017389-022-038-C07C-8409
GenBank Accession LN507017 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K017389-022-038-C07C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAAAAAACAGAGGAATCAGACACA
TGAGCACAGTTTGGAACTGAAAGAGAGACAAAAAACCAATCTTTTAGCTCAATTTACCTC
TTTTTGAAGACGTTTTTAGAATCTTTTCCATCGATCTCAAGGCTTCGCTTAAGGATGTTC
TTGTCTTGGTTGAAGCTCAACATGAAAATGGCATTCTGGTAAATACGGAGGATATCAGAG
ATCAAGGTTTTGTTGGAATCGACAGAAGAAGTGTGTTTGAGATTGTTCATCAATCGGTTA
GCAAGCTCTTTCCCTAGTTCCAATTCGTTGATCACAAGTTTCTCTTCCATCATCATCTTT
GCATTCACTTCAGAAAATTAAGAATCTTTTCTTAGATTTTCTTTTGAGTGAGATTGGTGG
GAGATGTGAGTTTCGTCTGTGAGTATCTAGATGTATTTATAGACATGAATGATGGAGATT
CTATGATGGTACTCGTCCTTGTGCTTAGACAATAAGTATTAATTTTTATTTTTGTATTTA
TTATTGGTTTCAAT
Primers used for PCR AJ06 - 8474, show primer details
   
Sequence Name 05-K105456-0022-038-C07C-AJ06
GenBank Accession LN488349 [GenBank]
Sequencing primer gene specific primer, AJ06: 5'-AAGATCCTTCTCTTTTCCACACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K105456-0022-038-C07C-AJ06
ATGAACATATAATACAAAATAAAAATTAATACTTATTGTCTAAGCACAAGGACGAGTACC
ATCATAGAATCTCCATCATTCATGTCTATAAATACATCTAGATACTCACAGACGAAACTC
ACATCTCCCACCAATCTCACTCAAAAGAAAATCTAAGAAAAGATTCTTAATTTTCTGAAG
TGAATGCAAAGATGATGATGGAAGAGAAACTTGTGATCAACGAATTGGAACTAGGGAAAG
AGCTTGCTAACCGATTGATGAACAATCTCAAACACACTTCTTCTGTCGATTCCAACAAAA
CCTTGATCTCTGATATCCTCCGTATTTACCAGAATGCCATTTTCATGTTGAGCTTCAACC
AAGACAAGAACATCCTTAAGCGAAGCCTTGAGATCGATGGAAAAGATTCTAAAAACGTCT
TCAAAAAGAGGTAAATTGAGCTAAAAGATTGGTTTTTTGTCTCTCTTTCAGTTCCAAACT
GTGCTCATGTGTCTGATTCCTCTGTTTTTTTTTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTTATT
   
Sequence Name 21-K105457-0022-038-C07C-8409
GenBank Accession LN495657 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K105457-0022-038-C07C-8409
TTTACATTGAAAAAAAACAGAGGAATCAGACACATGAGCACAGTTTGGAACTGAAAGAGA
GACAAAAAACCAATCTTTTAGCTCAATTTACCTCTTTTTGAAGACGTTTTTAGAATCTTT
TCCATCGATCTCAAGGCTTCGCTTAAGGATGTTCTTGTCTTGGTTGAAGCTCAACATGAA
AATGGCATTCTGGTAAATACGGAGGATATCAGAGATCAAGGTTTTGTTGGAATCGACAGA
AGAAGTGTGTTTGAGATTGTTCATCAATCGGTTAGCAAGCTCTTTCCCTAGTTCCAATTC
GTTGATCACAAGTTTCTCTTCCATCATCATCTTTGCATTCACTTCAGAAAATTAAGAATC
TTTTCTTAGATTTTCTTTTGAGTGAGATTGGTGGGAGATGTGAGTTTCGTCTGTGAGTAT
CTAGATGTATTTATAGACATGAATGATGGAGATTCTATGATGGTACTCGTCCTTGTGCTT
AGACAATAAGTATTAATTTTTATTTTTGTATTTATTATTGGTTTCAATCTTCGACCAGAA
GTTGTGTGGAAAA

Last Updated on 06.12.2018 16:11