Sequences from confirmation amplicon


Confirmation sequences for line 038F11 and hit At3g07160 are shown.
Line ID 038F11
Locus At3g07160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2272527
Primers used for PCR VL45 - 8474, show primer details
   
Sequence Name 25-K024065-022-038-F11C-8409
GenBank Accession LN497271 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K024065-022-038-F11C-8409
TAGATTTCTTATCCACAGTCTTTGAGTTGATAATCACATGCAAATATGAACAACAATAGT
GAGAAGAAATAACATGCCAGTCCCCTTGACTCAGCGGGGACAACATTTTCACGTACATTA
TTTAGGAAATTAGTGATTTATATAATGTAAAACAAGCCAACAGCTACATAAAAGAGAAGA
TGAAAAAAACATCACTACCTGATGAGAAGTATCAGACGTCCTGCACATGATATTGAATCA
TAGGCAAAAGAAATGAAAACGAGAAAATGTTAATATTTAACAAAGATAAATGGAGGAATA
CAGGAAAAATGATGTTCGTGGATAGGAGATGGAAAGGAACCTAACCGATTGGTAAGAGGA
ACAGGAAGAG
   
Sequence Name 26-K024065-022-038-F11C-VL45
GenBank Accession LN497747 [GenBank]
Sequencing primer gene specific primer, VL45: 5'-GCTCTTCATGTTCCTCTTACCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K024065-022-038-F11C-VL45
GTTCCTTTCCATCTCCTATCCACGAACATCATTTTTCCTGTATTCCTCCATTTATCTTTG
TTAAATATTAACATTTTCTCGTTTTCATTTCTTTTGCCTATGATTCAATATCATGTGCAG
GACGTCTGATACTTCTCATCAGGTAGTGATGTTTTTTTCATCTTCTCTTTTATGTAGCTG
TTGGCTTGTTTTACATTATATAAATCACTAATTTCCTAAATAATGTACGTGAAAATGTTG
TCCCCGCTGAGTCAAGGGGACTGGCATGTTATTTCTTCTCACTATTGTTGTTCATATTTG
CATGTGATTATCAACTCAAAGACTGTGGATAAGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATTC
Primers used for PCR VL44 - 8474, show primer details
   
Sequence Name 27-K024065-022-038-F11C-oriL
GenBank Accession LN498210 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K024065-022-038-F11C-oriL
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGATGCAGCACACTTTGC
TCCATTTTGGAACCAAATAATAAAAAGTTTACGAGAGGAAGACTACATCACTGATTTGTA
AGTGCTTTATGCTCTACAGTAACTATACTGTGTGCATAATTCTCTAAGCTTCTGCTATTA
TATATTTTTGTTTTTACTTATTTTTCTCATCTGGAATCATTTTTGTAGTGAGATGGAATT
GCTCCTGATGCCCAAGAATTCTGGTAGGCTCGAATTGGTTCAGTGGCCACTTTTTCTTCT
TTCCAGCAAGGTGAGTTTGTTACTTTACCTGCGTTTGTATTAAATTCAACGTTTTATTGT
GATATCTCGCTAGCCTCATTGCTTTCCATAAAATTTACATATGAGGTTACGTATGAGTGA
CAATTATTATTTGATAATCTACTGTAGATATTATTGGCCAAAGAGATTGCAGCTGAGAGT
AATTCGCAAGAGGAGATTCTGGAGAGGATTGAAAGGGATGACTATATGAAGTATGCTGTT
GAGG
   
Sequence Name 28-K024065-022-038-F11C-VL44
GenBank Accession LN498677 [GenBank]
Sequencing primer gene specific primer, VL44: 5'-TGTGATAGACTTCCTCAACAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K024065-022-038-F11C-VL44
TTCATATAGTCATCCCTTTCAATCCTCTCCAGAATCTCCTCTTGCGAATTACTCTCAGCT
GCAATCTCTTTGGCCAATAATATCTACAGTAGATTATCAAATAATAATTGTCACTCATAC
GTAACCTCATATGTAAATTTTATGGAAAGCAATGAGGCTAGCGAGATATCACAATAAAAC
GTTGAATTTAATACAAACGCAGGTAAAGTAACAAACTCACCTTGCTGGAAAGAAGAAAAA
GTGGCCACTGAACCAATTCGAGCCTACCAGAATTCTTGGGCATCAGGAGCAATTCCATCT
CACTACAAAAATGATTCCAGATGAGAAAAATAAGTAAAAACAAAAATATATAATAGCAGA
AGCTTAGAGAATTATGCACACAGTATAGTTACTGTAGAGCATAAAGCACTTACAAATCAG
TGATGTAGTCTTCCTCTCGTAAACTTTTTATTATTTGGTTCCAAAATGGAGCAAAGTGTG
CTGCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11