Sequences from confirmation amplicon


Confirmation sequences for line 039A05 and hit At1g76550 are shown.
Line ID 039A05
Locus At1g76550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28724127
Primers used for PCR RK79 - 8474, show primer details
   
Sequence Name 01-K021167-022-039-A05C-8409
GenBank Accession LN486393 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K021167-022-039-A05C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAAAAGAAAAAACAAAG
AAAAGTACCATGTCAAAGAATTAGAACCTGTTTCTTAATGAATGGAGGCAAAAACTCAAA
CAAAGCAGATGACCAAGATGACAACTGAGTAGAAATATTATCAACCTGCACACCCTCCTT
AAGCAATCCATGAATTTCCTGCATCGCACAACAACATGTAAATGCTTACTATGTTTTGGA
ACTCAAGAAACAATAAAAAATAGCACATTAAATGATAAATAGAAATGTTTGCCAGAATTG
AATCGTTACCTTCAACAGAGCATAGAGTTCAGGAATACTCTCAACAAGCCCTTCAGGAAT
TAGGATGACTCCATGATTTTTGTCTGATACAAATAGACAGTTTATCAATAAAGGTAGGGA
ACGAGCAACAGACATCACTTCCAACATTACAAAAAGACTAAAACCGAGTCCGAAATGGGA
CGTTTACGGCTTTGGAGTGATCCTTCTCGAGCTTCTCACGGGAAAGATTGTGTCTGTTGA
AGAGATTGTGCTAGGAAACGGACTTACGGTT
   
Sequence Name 05-K021167-022-039-A05C-RK79
GenBank Accession LN488166 [GenBank]
Sequencing primer gene specific primer, RK79: 5'-TTTTGGTATGTTGGAAGTGATGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K021167-022-039-A05C-RK79
GCTTGTTCCCTAACTTTATTGATAAACTGTCTATTTGTATCAGACAAAAATCATGGAGTC
ATCCTAATTCCTGAAGGGCTTGTTGAGAGTATTCCTGAACTCTATGCTCTGTTGAAGGTA
ACGATTCAATACTGGCAAACATTTCTATTTATCATTTAATGTGCTATTTTTTATTGTTTC
TTGAGTTCCAAAACATAGTAAGCATTTACATGTTGTTGTGCGATGCAGGAAATTCATGGA
TTGCTTAAGGAGGGTGTGCAGGTTGATAATATTTCTACTCAGATGTCATCTTGGACATCT
GCTTTGTTTGAGTTTTTGCCTCCATTCATTAAGAAACAGGTTCTAATTCTTTGACATGGT
ACTTTTCTTTGTTTTTTCTTTTGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATT
ACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTA
CATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAA
TTATTCTAATTCGGAAATCTTTATTTCGACGTGT
Primers used for PCR FL80 - 8474, show primer details
   
Sequence Name 43-K029744-022-039-A05C-8409
GenBank Accession LN504725 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K029744-022-039-A05C-8409
CATTTACAATTGAATATATCCTCCTTTACATGTAGGTTCGAGTATGTGTTCTTATGATAA
ATTGTCTTCTTTGTTTCTGCAGCTACTTCTCCATCCCGAGTCTGATGATTCTGCTCAGCT
ATCCCAGGTAAGAAGAAAGAAAATGATGGAGTTACTGTGCTATAAAAAGTTGGAATTCTA
ATTGCTCATTTACTTTCTTCTTAGATTGAGACGGAGAAACTTCTGGCTTATCTTGTGGAG
ACTGAAATGAACAAGCGCTTGGTATGTTGCACATCTTTTTCACTTGTCACAATCCATTTT
TTTCAAATTTTTTTGAATAACCTTGCCTGAATATTCAAGTTGTCCCTAAAACTAAGTTTT
GAAAATGACAGAAAGAAGGAACCTACAAAGGGAAGAAATTCAACGTTTATCTG
   
Sequence Name 44-K029744-022-039-A05C-FL80
GenBank Accession LN505067 [GenBank]
Sequencing primer gene specific primer, FL80: 5'-GACAGATAGCGTTGAATTTCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K029744-022-039-A05C-FL80
TTTTCAAACTTAGTTTTAGGGACAACTTGAATATTCAGGCAAGGTTATTCAAAAAAATTT
GAAAAAAATGGATTGTGACAAGTGAAAAAGATGTGCAACATACCAAGCGCTTGTTCATTT
CAGTCTCCACAAGATAAGCCAGAAGTTTCTCCGTCTCAATCTAAGAAGAAAGTAAATGAG
CAATTAGAATTCCAACTTTTTATAGCACAGTAACTCCATCATTTTCTTTCTTCTTACCTG
GGATAGCTGAGCAGAATCATCAGACTCGGGATGGAGAAGTAGCTGCAGAAACAAAGAAGA
CAATTTATCATAAGAACACATACTCGAACCTACATGTAAATGGAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11