Sequences from confirmation amplicon


Confirmation sequences for line 039B06 and hit At4g15370 are shown.
Line ID 039B06
Locus At4g15370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8779179
Primers used for PCR BB02 - 8474, show primer details
   
Sequence Name 27-K025301-022-039-B06C-8409
GenBank Accession LN498221 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K025301-022-039-B06C-8409
CCGGACATGAAGCCATTTACAATTGAATATGGAACCAGCGTTTTCAGCAGGCCAATGTCC
ATCATCAGCTTGCAATGCCGTGAAATATAGTAATCCTCTTCTTAATGCAGTCTTTGCATC
TTCATACGTTATTTTTTCTGCATCCTCCACTATCACTCGCGGAATCTTCTGCTCAAACTT
CTTCTCCCTAAGAAACTGTAAAGTCCACAAAATAAAACAAAAAGAATAAATTAAAATACT
ATTTTCTGAAATAAAGACCAAATTGTTATGTTTTACACTGTAAAATATTTCACCTGCATC
CTCCATAGGAGATCGGCACTAGCCTTGAACCGTGACCTGTTGTTGGAGAAATTCCGACGA
GCCTCCTCGACCTCGGCAAGTTCCTCCGGAGAGCCAGCGTTGGCATCAAACTCCCAAATC
TGCCTACCAACATAGTTATTGGTGGTGAACAAGTGAGTATTATCTTTGGCCTTAGCACCA
ATTCTCAGTCTCCACATTTTCTATCAATCGTTCAAGT
   
Sequence Name 28-K025301-022-039-B06C-BB02
GenBank Accession LN498688 [GenBank]
Sequencing primer gene specific primer, BB02: 5'-CATTCCGAATAAACTTGAACGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K025301-022-039-B06C-BB02
AGAAAATGTGGAGACTGAGAATTGGTGCTAAGGCCAAAGATAATACTCACTTGTTCACCA
CCAATAACTATGTTGGTAGGCAGATTTGGGAGTTTGATGCCAACGCTGGCTCTCCGGAGG
AACTTGCCGAGGTCGAGGAGGCTCGTCGGAATTTCTCCAACAACAGGTCACGGTTCAAGG
CTAGTGCCGATCTCCTATGGAGGATGCAGGTGAAATATTTTACAGTGTAAAACATAACAA
TTTGGTCTTTATTTCAGAAAATAGTATTTTAATTTATTCTTTTTGTTTTATTTTGTGGAC
TTTACAGTTTCTTAGGGAGAAGAAGTTTGAGCAGAAGATTCCGCGAGTGATAGTGGAGGA
TGCAGAAAAAATAACGTATGAAGATGCAAAGACTGCATTAAGAAGAGGATTACTATATTT
CACGGCATTGCAAGCTGATGATGGACATTGGCCTGCTGAAAACGCTGGTTCCATATTCAA
TTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAAT
ATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC
AGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATT
TATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATNTCGA
Primers used for PCR FL82 - 8474, show primer details
   
Sequence Name 75-K029747-022-039-B06C-8409
GenBank Accession LN513163 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K029747-022-039-B06C-8409
GCCATTTACAATTGAATATATCCTGCTCCCAACACTAAAATTTTACATTTATAGTTAAAA
CATGAATTATCGATCCAACAATGATTGAAAAAATAAAATCATGGTGCATAGGTGATATGT
CTGTACATCACTGGACATCTTGAGAAAATCTTCACTCACGAGCATCGTGTAGAGTTACTT
CGGTACATGTACAACCATCAGGTACATCTATAAATATCTCGTGTGCACACACATACATAG
GGTGCAAATATTGTGTGTAAATGAATGACCAGAAACAACTTAGTCGACAAAAAAAAAAGT
TTATTATTCAACTCATTCCTCTGCGAAATAGTAAATAAATTAATGAGTTGAACAATTTTC
TTTTGTATTTTGTTTATTTTCCCAATTATTGTTGGATGAGTTGAATCATTTTTACACTTA
TTCAACTCAAATGATGAAAAATTAGATGAATGGTCGTTAAACTCTTTCAA
   
Sequence Name 76-K029747-022-039-B06C-FL82
GenBank Accession LN513319 [GenBank]
Sequencing primer gene specific primer, FL82: 5'-TGAAAATTGTGGACTTGTTGAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K029747-022-039-B06C-FL82
TATTTTTCTCATTTGAGTTGATAAGTGTAAAAATGATTCAACTCATCCAACAATAATTGG
GGAAAATAAACAAAATACAAAAGAAAATTGTTCAACTCATTAATTTATTTACTATTTCGC
AGAGGAATGAGTTGAATAATAAACTTTTTTTTTTGTCGACTAAGTTGTTTCTGGTCATTC
ATTTACACACAATATTTGCACCCTATGTATGTGTGTGCACACGAGATATTTATAGATGTA
CCTGATGGTTGTACATGTACCGAAGTAACTCTACACGATGCTCGTGAGTGAAGATTTTCT
CAAGATGTCCAGTGATGTACAGACATATCACCTATGCACCATGATTTTATTTTTTCAATC
ATTGTTGGATCGATAATTCATGTTTTAACTATAAATGTAAAATTTTAGTGTTTGGGAGCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGTTAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTA

Last Updated on 06.12.2018 16:11