Sequences from confirmation amplicon


Confirmation sequences for line 039D05 and hit At3g12460 are shown.
Line ID 039D05
Locus At3g12460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3955468
Primers used for PCR TH03 - 8474, show primer details
   
Sequence Name 23-K024286-022-039-D05C-TH03
GenBank Accession LN496400 [GenBank]
Sequencing primer gene specific primer, TH03: 5'-CTAGCCTCACTCCTGGATAACCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K024286-022-039-D05C-TH03
CAATCTTCTCAAGCGAATAATTATACATCGATCTCCGTCCACTCGAATCTGAAACAAATT
CCCTCAAATCCATAAGCTCCGCAATCTCCAACTGATGTCTAGATCTCTCTAGCTTTCCTG
CATCTTGACTATTCCAAATTCCGACGAACGTGTTATCTCGATCCGCGAGGAAATTACGGA
GGACCTGCGGGACGCGTTCACAGTAACGGAGTTGGATGATGATACATCGATTACCAACGC
ATAGCTGCAGAGTATCCGCCGGAGGATCGGAGCTATAGAAAACCGGTGGAGAATCGGAGC
GATAACTGTCCGGAGAAGAATCGGAACGATAACTAACCGGTGGAGAAGCAGGGTGGTAAC
CAGAGGGTGTCCACTGAACGCCGACTCCAACGACGAGAGGGTGAGAAGAGAGACGGTTGT
GGAAGAAGACGTCGTGAATCCATTGGCCGATGACGGAAGGATCCGGCGTTACGGTGACGA
AAAGCTCTTCTCCAAAGAAATCGACTGAGTACCTTTGCTGAGTAGCGTAAGATCCGATGG
TTCTGATTGTCGGAGCCATTGATTTTGCCTTCAGTTTTTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAGAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTA
   
Sequence Name 24-K024286-022-039-D05C-8409
GenBank Accession LN496840 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K024286-022-039-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAAAAACTGAAGGCAAAATCAATGGCT
CCGACAATCAGAACCATCGGATCTTACGCTACTCAGCAAAGGTACTCAGTCGATTTCTTT
GGAGAAGAGCTTTTCGTCACCGTAACGCCGGATCCTTCCGTCATCGGCCAATGGATTCAC
GACGTCTTCTTCCACAACCGTCTCTCTTCTCACCCTCTCGTCGTTGGAGTCGGCGTTCAG
TGGACACCCTCTGGTTACCACCCTGCTTCTCCACCGGTTAGTTATCGTTCCGATTCTTCT
CCGGACAGTTATCGCTCCGATTCTCCACCGGTTTTCTATAGCTCCGATCCTCCGGCGGAT
ACTCTGCAGCTATGCGTTGGTAATCGATGTATCATCATCCAACTCCGTTACTGTGAACGC
GTCCCGCAGGTCCTCCGTAATTTCCTCGCGGATCGAGATAACACGTTCGTCGGAATTTGG
AATAGTCAAGATGCAGGAAAGCTAGAGAGATCTAGACATCAGTTGGAGATTGCGGAGCTT
ATGGATTTGAGGGAATTTGTTTCAGATTCGAGTGGACGGAGATCGATGTATAATTATTCG
CTTGAGAAGATTGTTGAGGAAAA
Primers used for PCR 38xa - 3144, show primer details
   
Sequence Name 74-K118835-0022-039-D05C-38xa
GenBank Accession LN513154 [GenBank]
Sequencing primer gene specific primer, 38xa: 5'-CGATTGAGTCGTATGTTTGCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K118835-0022-039-D05C-38xa
AGTTGCTGCTATCTTGTGATTTGAATTTGTTGTTGTTCTTCTCTCTTTATCAATCAACGT
CTTCTTCTAATTCATGAAAGTTTTCATGTTATCGTATTTTGTGACTATGAATGTTGACTG
GAAAATTAGTACATGTAGACTACGTTTGTAATAAAGGCATTGATATTAAAAGACTATCTA
ACGTTTTGATTAAAAACAAATACGCTTTGATGAATTATATTATCAATTTTGTTTAATTTG
ACATGGGACAAAAAAAATCACGACGTACCAAATTGGTCAATCAAATGTTTTTGCACCCAG
TTAAATGATTTCCGATTTTTAAACTGGATATAACCTCAAGCAAGATCAAGGATACGAGTT
TACAACTGAGATGCGTTTATCAATCCTAATCTTGATTTAGGATAATACTAATTTTTAGGA
GTATATAACATTAGCTAACGTAATCTGATAAACTATACTCAAAATATTCTCCATAATTCA
AATTTCGAGATCTTTATAAGAAATTCTTTTTGAATTCAAATCAGTAATAACCATTAAGGT
TTGGCGTAGGTTAATGACGTCACACTGCATCTCAACCGTCCAAATTTAACTGCATTTATT
TTCAAAAAGATCAATGGTGATAGCGAGCACCAGTACTACAATTTAACTAGCCTCCGACGA
TTGCAAAAGGAGAAGAAAGAAAGAGTAATTACCAAATTGAAAAGAAACAAAACATGAAAG
AAAGAGTAATTACCAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGG
   
Sequence Name 50-K118834-0022-039-D05C-35St
GenBank Accession LN507328 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K118834-0022-039-D05C-35St
CTTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGGTAATTACTCTTTCTTTCATGTTTTGTTTCTTTTCAATTTGGTAATTA
CTCTTTCTTTCTTCTCCTTTTGCAATCGTCGGAGGCTAGTTAAATTGTAGTACTGGTGCT
CGCTATCACCATTGATCTTTTTGAAAATAAATGCAGTTAAATTTGGACGGTTGAGATGCA
GTGTGACGTCATTAACCTACGCCAAACCTTAATGGTTATTACTGATTTGAATTCAAAAAG
AATTTCTTATAAAGATCTCGAAATTTGAATTATGGAGAATATTTTGAGTATAGTTTATCA
GATTACGTTAGCTAATGTTATATACTCCTAAAAATTAGTATTATCCTAAATCAAGATTAG
GATTGATAAACGCATCTCAGTTGTAAACTCGTATCCTTGATCTTGCTTGAGGTTATATCC
AGTTTAAAAATCGGAAATCATTTAACTGGGTGCAAAAACATTTGATTGACCAATTTGGTA
CGTCGTGATTTTTTTTGTCCCATGTCAAATTAAACAAAATTGATAATATAATTCATCAAA
GCGTATTTGTTTTTAATCAAAACGTTAGATAGTCTTTTAATATCAATGCCTTTATTACAA
ACGTAGTCTACATGTACTAATTTTCCAGTCAACATTCATAGTCACAAAATACGATAACAT
GAAAACTTTCATGAATTAGAAGAAGACGTTGATTGATAAAGAGAGAAGAACAACAACAAA
TTCAAATCACAAGATTAGCAGCAACTTTTAA

Last Updated on 06.12.2018 16:11