Sequences from confirmation amplicon


Confirmation sequences for line 040A09 and hit At3g60130 are shown.
Line ID 040A09
Locus At3g60130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22210541
Primers used for PCR VL08 - 8474, show primer details
   
Sequence Name 57-K017367-022-040-A09C-VL08
GenBank Accession LN509262 [GenBank]
Sequencing primer gene specific primer, VL08: 5'-AGTAAAATAAAGTTGCTTGGGGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K017367-022-040-A09C-vl08
ATTTATAGATAACATTATATCAAAATCAAAACATAGTCCCTTTCTTCTATAAAGGTTTTT
TCACAACCAAATTTCCATTATAAATCAAAAAATAAAAACTTAATTAGTTTTTACAGAAGA
AAAGAAAACAATGAGAGGTAAATTTCTAAGTTTACTGTTGCTCATTACTTTGGCCTGCAT
TGGAGTTTCCGCCAAGAAGCATTCCACAAGGCCTAGATTAAGAAGAAATGATTTCCCACA
AGATTTCGTTTTTGGATCTGCTACTTCTGCTTATCAGGTTTAGTTCTTATTCTTTTGTCC
ATTGGTTCTTTTTTGTATGTATGTTAACAATTGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCT
AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGG
   
Sequence Name 61-K017367-022-040-A09C-8409
GenBank Accession LN510391 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K017367-022-040-A09C-8409
CAATTGTTAACATACATACAAAAAAGAACCAATGGACAAAAGAATAAGAACTAAACCTGA
TAAGCAGAAGTATCAGATCCAAAAACGAAATCTTGTGGGAAATCATTTCTTCTTAATCTA
GGCCTTGTGGAATGCTTCTTGGCGGAAACTCCAATGCAGGCCAAAGTAATGAGCAACAGT
AAACTTAGAAATTTACCTCTCATTGTTTTCTTTTCTTCTGTAAAAACTAATTAAGTTTTT
ATTTTTTGATTTATAATGGAAATTTGGTTGTGAAAAAACCTTTATAGAAGAAAGGGACTA
TGTTTTGATTTTGATATAATGTTATCTATAAATAGAGTTGTTGGAGGGAGAAAGGGTGTA
GCCCCAAGCAACTTTATTTTAC

Last Updated on 06.12.2018 16:11