Sequences from confirmation amplicon


Confirmation sequences for line 040C05 and hit T12G13 are shown.
Line ID 040C05
Locus T12G13 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:5180444
Primers used for PCR EV47 - 8474, show primer details
   
Sequence Name 87-K037327-022-040-C05C-EV47
GenBank Accession LN514725 [GenBank]
Sequencing primer gene specific primer, EV47: 5'-TTTGCATGTTTTACACATCCTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K037327-022-040-C05C-EV47
TTTCTCCATCTTTCAAGCATTCACCATCATTTTTGTACATTTTGCATCTTTAGTCGAGTG
TAGGACTAGTCTTGCATACATTCTGCATTCTGGAGTTGTTTCAGGTGTTTTGGAGCTATT
GGTGCTCAGAAAGAGGAAAAACAGAACAGAAAGTCACACTTCCACCAACATACTCGATCA
CCAATAGCAGAGAAGGAAGCAGTCAGCTCAGCAGTCCATCCAGACACTTCTACTCGATCG
CAGAGGATAGCAGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGAT
AAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAA
TTCGGAAATCTTTATTTCGACGTGTCTACATTC
   
Sequence Name 94-K037327-022-040-C05C-8409
GenBank Accession LN515285 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>94-K037327-022-040-C05C-8409
GAGCCATTTCAATTGAATATATCCTGCCTGCTATCCTCTGCGATCGAGTACAAGTGTCTG
GATGGACTGCTGAGCTGACTGCTTCCTTCTCTGCTATTGGTGATCGAGTATGTTGGTGGA
AGTGTGACTTTCTGTTCTGTTTTTCCTCTTTCTGAGCACCAATAGCTCCAAAACACCTGA
AACAACTCCAGAATGCAGAATGTATGCAAGACTAGTCCTACACTCGACTAAAGATGCAAA
ATGTACAAAAATGATGGTGAATGCTATGAAAGATGGATGAAACTAGACTCAAAATGGTGA
TAAAAGGATGTGTAAAACATGCAAAAACCTGTCATTAATTTATTACCC

Last Updated on 06.12.2018 16:11