Sequences from confirmation amplicon


Confirmation sequences for line 042C03 and hit At2g46680 are shown.
Line ID 042C03
Locus At2g46680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:19167050
Primers used for PCR JH01 - 8474, show primer details
   
Sequence Name 18-K020068-022-042-C03C-8409
GenBank Accession LN494120 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K020068-022-042-C03C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGCAATTGAATATAGCAA
GTGATGCCAAAATCCGCCCTATTGCTACCTGTCGACCCACAAATCGTTTACTCAAAAATG
AATAAAAAATTTACGATAAAGCAAACCCAAAGTTATATCTTATTATATGCTAAGGAGCCC
TCCCAAAAAAGAACAACAAATCACATTTTTATATAACTGTTAACATAATAATCTCAGCCT
CATCAACACACATATATAGATAGCCAACATCACACAAACATAGAGATTCCAAAAAATAAA
AATAAAGAAAACATAAATCCTCTGAGGAAAAATTCCGATGAGATGACAGAAGGTGGAGAA
TATTCTCCGGCGATGATGTCAGCAGAGCCATTCTTGACCATGAAGAAGATGAAGAAGAGC
AACCACAACAAGAACAATCAGAGAAGGTTTAGCGACGAGCAGATCAAGTCACTGGAGATG
ATGTTTGAGTCTGAGACAAGCTTGACCAAGAAGAAGTTCAATTAGCTAGAGAGCTAGGGT
TGCAGCCGAGGCAAGTGGCTATATGGTTTCAGAACAAGAGGGCTCGTTGGAAATCCAAGC
AGCTCGAGACTGAGTACAACATTCTCA
   
Sequence Name 17-K020068-022-042-C03C-JH01
GenBank Accession LN493674 [GenBank]
Sequencing primer gene specific primer, JH01: 5'-CCAAGTTGTCGTAGTTTTGTCTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K020068-022-042-C03C-JH01
GAATGTTGTACTCAGTCTCGAGCTGCTTGGATTTCCAACGAGCCCTCTTGTTCTGAAACC
ATATAGCCACTTGCCTCGGCTGCAACCCTAGCTCTCTAGCTAATTGAACCTTCTTCCTTG
GCTCAAGCCTTGTCTCAGACTCAAACATCATCTCCAGTGACTTGATCTGCTCGTCGCTAA
ACCTTCTCTGATTGTTCTTGTTGTGGTTGCTCTTCTTCATCTTCTTCATGGTCAAGAATG
GCTCTGCTGACATCATCGCCGGAGAATATTCTCCACCTTCTGTCATCTCATCGGAATTTT
TCCTCAGAGGATTTATGTTTTCTTTATTTTTATTTTTTGGAATCTCTATGTTTGTGTGAT
GTTGGCTATCTATATATGTGTGTTGATGAGGCTGAGATTATTATGTTAACAGTTATATAA
AAATGTGATTTGTTGTTCTTTTTTGGGAGGGCTCCTTAGCATATAATAAGATATAACTTT
GGGTTTGCTTTATCGTAAATTTTTATTCATTTTTGATAAACGATTTGTGGGTCGACAGGT
AGCAATAGGGCGGATTTTGGCATCACTTGCTATATTCAATTGCAGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTCATGGATCAACAATGAGTATGATGGTCAATATGGA
Primers used for PCR FL89 - 8474, show primer details
   
Sequence Name 33-K029744-022-042-C03C-8409
GenBank Accession LN500873 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K029744-022-042-C03C-8409
CATTTACAATTGATATAGCCAAGTCGTCGCTATCACTTGTTAAGTCGAGTTTCATTTCAA
CTCGAAACTAATCATCTAACTACTGATTAATAATTTCCACCTTTTTTATTTCTTTTACTT
GTGACAAAATTAGTTTCGACTTAACAAGTGTTAGAAAATTAAATGATACAAAAAAATCTG
CATACTACACAATTTACCGCATAGTAAACATTCTGGTCGATGTGAAAATGGAAATGGAAC
AACGGTTGCGTACCAATGTATTTAGTGATGTGGGTGCATATTAAC
   
Sequence Name 34-K029744-022-042-C03C-FL89
GenBank Accession LN501290 [GenBank]
Sequencing primer gene specific primer, FL89: 5'-TTGTTAATATGCACCCACATCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K029744-022-042-C03C-FL89
TTCATTTCATTTTCACATCGACCAGAATGTTTACTATGCGGTAAATTGTGTAGTATGCAG
ATTTTTTTGTATCATTTAATTTTCTAACACTTGTTAAGTCGAAACTAATTTTGTCACAAG
TAAAAGAAATAAAAAAGGTGGAAATTATTAATCAGTAGTTAGATGATTAGTTTCGAGTTG
AAATGAAACTCGACTTAACAAGTGATAGCGACGACTTGGCTATATCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11