Sequences from confirmation amplicon


Confirmation sequences for line 042G09 and hit At2g18570 are shown.
Line ID 042G09
Locus At2g18570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8064764
Primers used for PCR CW10 - 8474, show primer details
   
Sequence Name 46-K104195-0022-042-G09C-CW10
GenBank Accession LN505856 [GenBank]
Sequencing primer gene specific primer, CW10: 5'-CCAATTGTTAGGACTAACCAGCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K104195-0022-042-G09C-CW10
TTCGAGTGGCTGACGAGCAACGGGAAAGGTCAGTGGTGTTTGTGTGTTTAGGGAGCGGTG
GAACGTTGACGTTTGAGCAAACAGTGGAACTCGCTTTGGGTTTAGAGTTAAGTGGTCAAA
GGTTCGTTTGGGTTCTACGTAGGCCCGCTTCATATCTCGGGGCGATCTCCAGCGATGATG
AACAGGTAAGTGCCAGTCTACCTGAAGGTTTCTTGGACCGCACGCGTGGTGTGGGGATTG
TGGTTACGCAATGGGCACCACAAGTTGAGATCTTGAGCCATAGATCGATCGGTGGGTTCT
TGTCTCACTGCGGTTGGAGTTCGGCTTTGGAAAGTTTGACTAAAGGAGTTCCGATCATCG
CTTGGCCTCTTTATGCGGAGCAGTGGATGAATGCCACGTTATTGACTGAGGAGATCGGTG
TGGCCGTTCGTACATCGGAGTTACCGTCGGAGAGAGTCATCGGAAGGGAAGAAGTGGCAT
CTCTGGTGAGAAAGATTATGGCGGAAGAGGATGAAGAAGGACAGAAAATTAGGGCTAAAG
CTGAGGAGGTGAGGGTTAGCTCCGGACAGGATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 62-K104196-0022-042-G09C-8409
GenBank Accession LN510749 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K104196-0022-042-G09C-8409
GAGCCATTTACAATTGAATATATCCTGTCCGGAGCTAACCCTCACCTCCTCAGCTTTAGC
CCTAATTTTCTGTCCTTCTTCATCCTCTTCCGCCATAATCTTTCTCACCAGAGATGCCAC
TTCTTCCCTTCCGATGACTCTCTCCGACGGTAACTCCGATGTACGAACGGCCACACCGAT
CTCCTCAGTCAATAACGTGGCATTCATCCACTGCTCCGCATAAAGAGGCCAAGCGATGAT
CGGAACTCCTTTAGTCAAACTTTCCAAAGCCGAACTCCAACCGCAGTGAGACAAGAACCC
ACCGATCGATCTATGGCTCAAGATCTCAACTTGTGGTGCCCATTGCGTAACCACAATCCC
CACACCACGCGTGCGGTCCAAGAAACCTTCAGGTAGACTGGCACTTACCTGTTCATCATC
GCTGGAGATCGCCCCGAGATATGAAGCGGGCCTACGTAGAACCCAAACGAACCTTTGACC
ACTTAACTCTAAACCCAAAGCGAGTTCCACTGTTTGCTCAAACGTCAACGTTCCACCGCT
CCCTAAACACACAAACACCACTGACCTTTCCCGTTGCTCGTCTAGCCACTCGAATATACN
ATTGGGTTTGTCTACATGCTGGTTAGTC

Last Updated on 06.12.2018 16:11