Sequences from confirmation amplicon


Confirmation sequences for line 043D04 and hit At4g29340 are shown.
Line ID 043D04
Locus At4g29340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14447483
Primers used for PCR CL48 - 8474, show primer details
   
Sequence Name 86-K033216-022-043-D04C-CL48
GenBank Accession LN514605 [GenBank]
Sequencing primer gene specific primer, CL48: 5'-ACTGAACTCCTGTCCCTTGAACTA-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K033216-022-043-D04C-CL48
GAGACAACATGCATCATTTGTTTTCAATCTATGCAAAACATATAAAAAGAAACACACACA
CACAAAATACACTATATATATATCCCATGACAAACATTGATAGAATCTAATCAAAACATG
ATGCATGATCTAAATCACATTGTAAAAACACATATCATATCGGATAGCAACACTATCAGG
ATCTAAATCACATTGAGATCAGACGCAATGAAATCAAATACCTGAGGGAAGTTGGCGCTC
TGAGCCCAAACGCTACCGTCATGACCAACGATAGCGGCAGCAGTAAGGTGGTGTCCTTGA
CCATCGCCAACATCACACATCAAATGCTCATCAACGTAAGTTTGCCACGACATCTTCTTC
TTCTTTTCTTATTTTTTTTCTTCTTCTTTGTTTATGATCTATACGTTAAGTGCCCCGGTT
ATGTGCAGCGTGGGGACTTCGTCCTTCAGATAAATAGGAGAGGAAGGAACAAGGATCATT
TTAAAACCGTTGGATCACATAGACGAATTCTCAGCCCTACATCTTACTTTTTCTTCAAAA
AAACAAGGAGTGATTTTCAATTAGTGTAGCTCAAGTTTCGCCTTTGTTATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
TTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATA
GGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT
   
Sequence Name 83-K033216-022-043-D04C-8409
GenBank Accession LN514256 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K033216-022-043-D04C-8409
GCCATTTACAATTGAATAAACAAAGGCGAAACTTGAGCTACACTAATTGAAAATCACTCC
TTGTTTTTTTGAAGAAAAAGTAAGATGTAGGGCTGAGAATTCGTCTATGTGATCCAACGG
TTTTAAAATGATCCTTGTTCCTTCCTCTCCTATTTATCTGAAGGACGAAGTCCCCACGCT
GCACATAACCGGGGCACTTAACGTATAGATCATAAACAAAGAAGAAGAAAAAAAATAAGA
AAAGAAGAAGAAGATGTCGTGGCAAACTTACGTTGATGAGCATTTGATGTGTGATGTTGG
CGATGGTCAAGGACACCACCTTACTGCTGCCGCTATCGTTGGTCATGACGGTAGCGTTTG
GGCTCAGAGCGCCAACTTCCCTCAGGTATTTGATTTCATTGCGTCTGATCTCAATGTGAT
TTAGATCCTGATAGTGTTGCTATCCGATATGATATGTGTTTTTACAATGTGATTTAGATC
ATGCATCATGTTTTGATTAGATTCTATCAATGTTTGTCATGGGATATATATATAGTGTAT
TTTGTGTGTGTGTGTTTCTTTTTATATGTTTTGCATAGATTTGAAAACAAATGATGCATG
TTGTCTCATGGTAATCGACTAATCGTGATTTGGTCTTTCATATAGTTCAAGGGACAGGAG
TTCA

Last Updated on 06.12.2018 16:11