Sequences from confirmation amplicon


Confirmation sequences for line 044H05 and hit At3g42624 are shown.
Line ID 044H05
Locus At3g42624 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14714622
Primers used for PCR EV55 - 8474, show primer details
   
Sequence Name 05-K103223-0022-044-H05C-8409
GenBank Accession LN488312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K103223-0022-044-H05C-8409
ATGGAACGTAATCGTCCATACTCTCTTGAGTATAAGCAGGGGCTGAAGTTGAGGCGGGAG
GATTTTGCTCGTTCTGTCCGGTCGAGCGTATGGAAGCTCGTCTCTGCCTACTTCTTGGCT
CTCTCAACTCGGAGGTCACTTCCCGAGGCTCAAAAGATAAAGCTCGGGGTTGATCGATCC
TTTCAGCTCTACTTGTAGCTGAGCTCGTGGAACCGCTTCTTCTTACTTCGGGGGTGGGAG
AGAATGGCCTAGGCTTGCTCTGGAAGTCCTTGATTTGAGCAGCCATACTCTTCAACGACT
TCTTCATTGTTCTCATCTTCTTCTTGAAACCCTTCATTGTTTTGCTGTGAAGGTTCCCAA
GCTCTTCAATCACACTGAGCTTCTTATGAACTTCAGCTATCTCATCTCCTCCTTGGTTAA
CACCTTCTTCATAATGAGCTTGCTCTTGTTCGCTGGTCCCTTGTTCCGGTTGATCGTTAT
GTTGCTCAAACTCGATTTGCTGCTCATAGTCAGCTTGAGTAAAATACTCTTGTTGCTCAT
   
Sequence Name 53-K103230-0022-044-H05C-EV55
GenBank Accession LN508120 [GenBank]
Sequencing primer gene specific primer, EV55: 5'-GTCAAGCTGACTATGAGCAACAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K103230-0022-044-H05C-EV55
AATCGAGTTTGAGCAACATAACGATCAACCGGAACAAGGGACCAGCGAACAAGAGCAAGC
TCATTATGAAGAAGGTGTTAACCAAGGAGGAGATGAGATAGCTGAAGTTCATAAGAAGCT
CAGTGTGATTGAAGAGCTTGGGAACCTTCACAGCAAAACAATGAAGGGTTTCAAGAAGAA
GATGAGAACAATGAAGAAGTCGTTGAAGAGTATGGCTGCTCAAATCAAGGACTTCCAGAG
CAAGCCTAGGCCATTCTCTCCCACCCCCGAAGTAAGAAGAAGCGGTTCCACGAGCTCAGC
TACAAGTAGAGCTGAAAGGATCGATCAACCCCGAGCTTTATCTTTTGAGCCTCGGGAAGT
GACCTCCGAGTTGAGAGAGCCAAGAAGTAGGCAGAGACGAGCTTCCATACGCTCGACCGG
ACAGAACGAGCAAAATCCTCCCGCCTCAACTTCAGCCCCTGCTTATACTCAAGAGAGTAT
GGACGATTACGTTTCCATCTACTTCACCTGACCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACNAATTAC
GATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
NTCGACGTGTCTA

Last Updated on 06.12.2018 16:11