Sequences from confirmation amplicon


Confirmation sequences for line 045H01 and hit At1g70700 are shown.
Line ID 045H01
Locus At1g70700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:26656568
Primers used for PCR EV56 - 8474, show primer details
   
Sequence Name 10-K037446-022-045-H01C-8409
GenBank Accession LN490565 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K037446-022-045-H01C-8409
CCATTTACATTGAATATATCCTGCAAAAAGCAATTTTCGAGTTTAGACATATGATTTGAG
GAAGCAAAAATCTTGATTATATTTTGTTTTGATGTTGATATTAGACACACATACCTGAAG
GAATCATGTTACGAGGAGCTGCATTGAATGATTCGATCATGCTCATTGCATTAGTCGCAG
CAACGGGTGTGTCCCTACACCTTGAGAAATTATCAGTGTGAGTGGCAGAGCTTGATGAGG
TAGCAGCAGTGTTATGGATTTGTTTTCCATACATTCTTTCAGCTTCTCGAACCGGTTTGC
TATCTCCAGTTTCACCTTTCAAACCGTTCCCGGCGCATAACATGATGGCTTGAGCCTACT
CACATATCATGAATAGTCCGTTTTAGAACATGTAAATGTTATGTAAATGTTATTTGTATG
AATCTTGATTATATATACCTTATCGGGAGATATGTCATTAAAGACGCTTATAGTTCCGCC
ATAAAAGATTGTGAGCTGAGGAGATGATCCGGAGGAAGCCACTCTGTTTCCACAAACCCA
CATAACCAAATCAAAATATGAAAAGAGCAAAAAAATTGTTGAATGTTTTTGCATCATAAT
ATGGAAATTACCGTGGAACAAGAGAAGGATGAGTTGGTAAAGGAACTGACGCC
   
Sequence Name 02-K037446-022-045-H01C-EV56
GenBank Accession LN486942 [GenBank]
Sequencing primer gene specific primer, EV56: 5'-TTACTAGGCGGTTCAGTTCCTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K037446-022-045-H01C-EV56
TATTTCCATATTATGATGCAAACATTCAACAATTTTTTTGCTCTTTTCATATTTTGATTT
GGTTATGTGGGTTTGTGGAAACAGAGTGGCTTCCTCCGGATCATCTCCTCAGCTCACAAT
CTTTTATGGCGGAACTATAAGCGTCTTTAATGACATATCTCCCGATAAGGTATATATAAT
CAAGATTCATACAAATAACATTTACATAACATTTACATGTTCTAAAACGGACTATTCATG
ATATGTGAGTAGGCTCAAGCCATCATGTTATGCGCCGGGAACGGTTTGAAAGGTGAAACT
GGAGATAGCAAACCGGTTCGAGAAGCTGAAAGAATGTATGGAAAACAAATCCATAACACT
GCTGCTACCTCATCAAGCTCTGCCACTCACACTGATAATTTCTCAAGGTGTAGGGACACA
CCCGTTGCTGCGACTAATGCAATGAGCATGATCGAATCATTCAATGCAGCTCCTCGTAAC
ATGATTCCTTCAGGTATGTGTGTCTAATATCAACATCAAAACAAAATATAATCAAGATTT
TTGCTTCCTCAAATCATATGTCTAAACTCGAAAATTGCTTTTTTGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTNTATNTCGACGTGTCTAC

Last Updated on 06.12.2018 16:11