Sequences from confirmation amplicon


Confirmation sequences for line 049C10 and hit MEK6 are shown.
Line ID 049C10
Locus MEK6 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7709068
Primers used for PCR FN27 - 3144, show primer details
   
Sequence Name 58-K032734-0022-049-C10C-FN27
GenBank Accession LN509616 [GenBank]
Sequencing primer gene specific primer, FN27: 5'-TGAAGATGTTTCACTTGCAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K032734-0022-049-C10C-FN27
GTTTTGAATCCTTAACCTTATTTTAAATTAAAATGTTGTTTTTACTAAGTTAAGAAAAGT
TAGGAGGAAGTTTAACTAATTTCTATTTCAATGGTTAAGAACTATTGGATTCTTAAAAAA
CAACAATTCAAAAATGTCTATCCACTCAAAATACAACACATATACTCTTCTTAGTTTTAA
GAAAACATCTTAATTAAGATGTTTATCTTAGCTAAGGTTTGTTGTTTCATTAAATTTAAT
CACTTTTATTGCTAAGAATACAAGGCTGGAGTTGCTTTTATGTACCTTTAAATTAAGGAA
AATGACAAGTCCAAAGAAGTAGTGAGAAAGATTTACTACACTAAAATAAATATCTAACAA
AATACCAAACAGAGAGTTTGGTGAGTTAATTTTTTTTTACAGCAGTTTTATGGAACTTTT
TGTTTGTTGGTTACAAACATTGCTTAGTAGAAAAAAGCAAAAGATCAAACACTGATAGTT
TAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAA
TGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCT
TACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTC
TTTTTCCACGATGCTCCT
   
Sequence Name 26-K032734-0022-049-C10C-3144
GenBank Accession LN497811 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K032734-0022-049-C10C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATCTTTTGCT
TTTTTCTACTAAGCAATGTTTGTAACCAACAAACAAAAAGTTCCATAAAACTGCTGTAAA
AAAAAATTAACTCACCAAACTCTCTGTTTGGTATTTTGTTAGATATTTATTTTAGTGTAG
TAAATCTTTCTCACTACTTCTTTGGACTTGTCATTTTCCTTAATTTAAAGGTACATAAAA
GCAACTCCAGCCTTGTATTCTTAGCAATAAAAGTGATTAAATTTAATGAAACAACAAACC
TTAGCTAAGATAAACATCTTAATTAAGATGTTTTCTTAAAACTAAGAAGAGTATATGTGT
TGTATTTTGAGTGGATAGACATTTTTGAATTGTTGTTTTTTAAGAATCCAATAGTTCTTA
ACCATTGAAATAGAAATTAGTTAAACTTCCTCCTAACTTTTCTTAACTTAGTAAAAACAA
CATTTTAATTTAAAATAAGGTTAAGATTCAAAACTAAGAACCAATTGGTTCTAAACCAGT
GGGGTAATAACTAAACTTTTT
Primers used for PCR IL23 - 8474, show primer details
   
Sequence Name 16-K032413-0022-049-C10C-8409
GenBank Accession LN493327 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K032413-0022-049-C10C-8409
GAGCCTTTGCTAGTAGAAAACTCAATAACAACAAACGATCCTTGTTTCTTCCCTTTCTAT
TGCAGATATTTTTTGCTATTTTTAGTCACTACCCATTTCTTAAAATTCTTTTTTTTTTTC
CATTACACATTTCTAAAAATTCTAAGCTTCAAAAAATTAAAATTTTCAAATTTTTCTTAA
AAAAAAATTGCATAAAACCCCTAAAATATTTATAGCTTACCCCAAAAAAATCTAATAATC
AAAAAAAAACAAACGGAACCCCAGGATTATCTGGTTCACTTCTATTCCCCACGGGGGTTT
CCCCAACCGTCGTCCCCGACGGTAAAACAACGGGG
   
Sequence Name 16-K032413-0022-049-C10C-IL23
GenBank Accession LN493328 [GenBank]
Sequencing primer gene specific primer, IL23: 5'-GATCATGACTTCCACGTTGTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K032413-0022-049-C10C-IL23
TAGAAGTGAACAAGATAATCATGAGGTTCCGTTTGTTTTTCTCTGATTATTAGATTTTTT
TGGTGTAAGCTATAAATATTCTAGGGTTTTTATGCAATTTTCTTTTAAGAAAAATCTGAA
AATTTTAATTTTATGAAGCTTAGAATTTTAAGAAATGTGTAATGAAAAAAAAAAAAAAAT
TTTAAAAAAGGGGTAGGGACTAAAAATAGCAAAAAATATCTGCAATAAAAGGGGAAAAAA
CAGGGATCGTTTGTTGTTATTGAGTTATTCTACAAAGCAAAGGGCTTCATGTCCGGGAAA
TCTACATGGATCACCAATGAGTATGAGGGTCAATATGGAAAAAAAAAAAGAGTAATTACC
ATTTTTTTTTCAATTCAAAAATGTAAATGTCCCCACCGTTTTTATAAAATGAAAGTACTT
TTTGATAAAACGACAAATTACAATCCGTCGTATTTATGGGCAAAAGCAATAAACAAATTA
TTCTAATTCGAAAATCTTTATTTCAACGTGTCTACTTTCC

Last Updated on 06.12.2018 16:11