Sequences from confirmation amplicon


Confirmation sequences for line 050B11 and hit At5g64610 are shown.
Line ID 050B11
Locus At5g64610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:25828526
Primers used for PCR IL19 - 8474, show primer details
   
Sequence Name 17-K032414-022-050-B11C-8409
GenBank Accession LN493743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K032414-022-050-B11C-8409
TTACAATTGAATATATCCTGGCGAACATGACAGCGATTAAAGCAGAAGATATATTAAGCA
CCCTGCAGAGCTTGGAACTGATACAATACAGGAAAGGACAACACGTAATCTGCGCGGATC
CTAAGGTACTGGACCGACACTTGAAAGCGGCAGGCCGAGGTGGTCTTGATGTGGATGTGA
GCAAAATGATATGGACTCCTTACAAAGAGCAGAGCTAAGAGGGCATTACACTTCAAATAG
TTATGGTTATGGTCTACTTTTAATCTGCATATCCTTAGATGACTTTTGTCTGTAACAATT
TAACACGCACTCTGTTCAATAGATCTTGTAACTGTAGGAATCTTTTGTCTGGAATGGTAA
   
Sequence Name 17-K032414-022-050-B11C-IL19
GenBank Accession LN493744 [GenBank]
Sequencing primer gene specific primer, IL19: 5'-TTTACCATCCAGACAAAAGATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K032414-022-050-B11C-IL19
TTGTTACAGACAAAAGTCATCTAAGGATATGCAGATTAAAAGTAGACCATAACCATAACT
ATTTGAAGTGTAATGCCCTCTTAGCTCTGCTCTTTGTAAGGAGTCCATATCATTTTGCTC
ACATCCACATCAAGACCACCTCGGCCTGCCGCTTTCAAGTGTCGGTCCAGTACCTTAGGA
TCCGCGCAGATTACGTGTTGTCCTTTCCTGTATTGTATCAGTTCCAAGCTCTGCAGGGTG
CTTAATATATCTTCTGCTTTAATCGCTGTCATGTTCGCCAGGATATATTCAATTGTAAAT
GGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAA
AAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTAT
TATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCG
AAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC

Last Updated on 06.12.2018 16:11