Sequences from confirmation amplicon


Confirmation sequences for line 052D04 and hit At5g65080 are shown.
Line ID 052D04
Locus At5g65080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26001591
Primers used for PCR EV60 - 8474, show primer details
   
Sequence Name 25-K118126-0022-052-D04C-EV60
GenBank Accession LN497652 [GenBank]
Sequencing primer gene specific primer, EV60: 5'-TGTTTCCAAAATTTTCTTCAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K118126-0022-052-D04C-EV60
TTTTATAGGTAAACAACAAAATACTTAATATAACAGCAAGATCTAGACTTTTAAAGCATG
GTAATTTGTTTTTCAGTTCTTAAAATGATCTTTTCATGTGTTAGAAACTTTCTTACACTT
CTTTTTCTACATATATTAACTAAAATATAACATGAAACATAGAGTTTAAGAGCATGATTG
TCTTTTTTTGTTGCAAATTTAAAATATAATTGTGGGTTTCACCAGAAAGTGTGTAGTAGC
CATTAGAGAGTCCCTTAACTTTCATCTGTAAGCTTTTTAAACATGTTCTTTTGAAGTATG
TATTAATTAAAAAGCAGGTTTCTTTAACTTTCATACGTATGTATAGTATATGCAAGTGCA
TACGTATCTATCTCTATCTAATGTCTATCTATACGACGTGTAAAATGATTAAATTAGTTT
CTTTACGCTTAAGAGATTGTGCCTCTGTTACCTCGCTAGCTAGAACCTTGTTCTTTTCTT
TCAGCAACTTAAAAGAAACTTGGATCAGCCATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 09-K118125-0022-052-D04C-8409
GenBank Accession LN490389 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K118125-0022-052-D04C-8409
GCATTTCATGGCTGATCCAGTTTCTTTTAAGTTGCTGAAAGAAAAGAACAAGGTTCTAGC
TAGCGAGGTAACAGAGGCACAATCTCTTAAGCGTAAAGAAACTAATTTAATCATTTTACA
CGTCGTATAGATAGACATTAGATAGAGATAGATACGTATGCACTTGCATATACTATACAT
ACGTATGAAAGTTAAAGAAACCTGCTTTTTAATTAATACATACTTCAAAAGAACATGTTT
AAAAAGCTTACAGATGAAAGTTAAGGGACTCTCTAATGGCTACTACACACTTTCTGGTGA
AACCCACAATTATATTTTAAATTTGCAACAAAAAAAGACAATCATGCTCTTAAACTCTAT
GTTTCATGTTATATTTTAGTTAATATATGTAGAAAAAGAAGTGTAAGAAAGTTTCTAACA
CATGAAAAGATCATTTTAAGAACTGAAAAACAAATTACCATGCTTTAAAAGTCTAGATCT
TGCTGTTATATTAAGTATTTTGTTGTTTACCTATAAAAAATGAAAAGAAATTTTCTTACA
GGTGGGGAAGCTGAAGAAAATTTTGGAAAC

Last Updated on 06.12.2018 16:11