Sequences from confirmation amplicon


Confirmation sequences for line 052D06 and hit At5g55090 are shown.
Line ID 052D06
Locus At5g55090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22357002
Primers used for PCR 60aq - 8474, show primer details
   
Sequence Name 03-K106755-0022-052-D06C-8409
GenBank Accession LN487502 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K106755-0022-052-D06C-8409
GCCATTTACATTGATCATGTAAAAATAATAAATGTTGTCCTCAGGGGAATATTCCAAGAA
CAAGCTGTCTTGTTGATCCCAGATCCAATTCTCAAATCCTCCCACTTCTTCTTCGTCCTC
CTCCAATGACGTTGCTTCAACGCAATTCTCATCTTCTTCCTCGTTACGTTTCTCTATCTC
TCCCCTTACTTCAATCCAACCGTTCGTTTCCCAGTCTGGTTCACCGGAACTCTCGTCTCC
GGCGAGTTTCTTGATCCGATCACCCGGTAAAGAATCGTCATCCCATAAGAAATTTGTAGA
ATTTGCAAATGGGTCTTCGTGATCCTCTTTAATGAATCTACTTCTCGAAGTTTCACATAA
ATCCCAAAACCGTTGATCCAACACAGTACTAGGAGATGAAGAATTCAGACAGTTACCAGT
TTGGTCACTGTCGTTGTCTTCTTCGTCGAGAAACGGATGTTGAAGCAATTCTTCAACAGT
CCATCTCTGTTTCGGATCTTTTCTCAAACACTTCCTCAAAAAGTCTTGACCTTTCTCCGA
TAACCAAACCGGAATCACCGGCGACTCGCCGGTGAAACCAATCTTATAAATAGCAGCAAC
GACGTCGTTTAGCTCCGGCCAAGGACTTGACCCTGTA
   
Sequence Name 19-K106756-0022-052-D06C-60aq
GenBank Accession LN494776 [GenBank]
Sequencing primer gene specific primer, 60aq: 5'-GATAGAGATGGCTACAGGGTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K106756-0022-052-D06C-60aq
AAAGATTGGTTTCCCGGCGAGTCGCCGGTGATTCCGGTTTGGTTATCGGAGAAAGGTCAA
GACTTTTTGAGGAAGTGTTTGAGAAAAGATCCGAAACAGAGATGGACTGTTGAAGAATTG
CTTCAACATCCGTTTCTCGACGAAGAAGACAACGACAGTGACCAAACTGGTAACTGTCTG
AATTCTTCATCTCCTAGTACTGTGTTGGATCAACGGTTTTGGGATTTATGTGAAACTTCG
AGAAGTAGATTCATTAAAGAGGATCACGAAGACCCATTTGCAAATTCTACAAATTTCTTA
TGGGATGACGATTCTTTACCGGGTGATCGGATCAAGAAACTCGCCGGAGACGAGAGTTCC
GGTGAACCAGACTGGGAAACGAACGGTTGGATTGAAGTAAGGGGAGAGATAGAGAAACGT
AACGAGGAAGAAGATGAGAATTGCGTTGAAGCAACGTCATTGGAGGAGGACGAAGAAGAA
GTGGGAGGATTTGAGAATTGGATCTGGGATCAACAAGACAGCTTGTTCTTGGAATATTCC
CCTGAGGACAACATTTATTATTTTTACATGATCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11