Sequences from confirmation amplicon


Confirmation sequences for line 053C12 and hit At5g53530 are shown.
Line ID 053C12
Locus At5g53530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21746895
Primers used for PCR MJ63 - 8474, show primer details
   
Sequence Name 01-K029908-022-053-C12C-8409
GenBank Accession LN486446 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K029908-022-053-C12C-8409
ATTGAATATTGTACGCTTAATTCCATTGGACCCTTACCCATTGTACAGATGGAAGTTGGA
ATCGAGGACTGTCTGCATATCGAGTTTGAGTACAATAAAAGCAAGTAAGATAAACTGATT
TTCGTAACTTTGGGTTTCGAGCGATGTGGAAAATATAAAACAGTAGCTAATTCTGTATGC
TCTCGATGCAGGTATCACCTAAAAGATGTTATCCTTGGGAAAATATACTTTCTTCTGGTG
AGAATCAAGATAAAGAATATGGATCTTGAGATAAGACGGCGAGAATCAACTGGAGAGNGA
AGCTAAT
   
Sequence Name 01-K029908-022-053-C12C-MJ63
GenBank Accession LN486447 [GenBank]
Sequencing primer gene specific primer, MJ63: 5'-TATTAGCTCCTGCTCCAGTTGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K029908-022-053-C12C-MJ63
TTCTTATCTGATTCTCACCAGAAGAAGTATATTTTCCCAAGGATAACATCTTTTAGGTGA
TACCTGCATCGAGAGCATACAGAATTAGCTACTGTTTTATATTTTCCACATCGCTCGAAA
CCCAAAGTTACGAAAATCAGTTTATCTTACTTGCTTTTATTGTACTCAAACTCGATATGC
AGACAGTCCTCGATTCCAACTTCCATCTGTACAATGGGTAAGGGTCCAATGGAATTAAGC
GTACAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11