Sequences from confirmation amplicon


Confirmation sequences for line 053G03 and hit At3g02840 are shown.
Line ID 053G03
Locus At3g02840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:618443
Primers used for PCR AE01 - 8474, show primer details
   
Sequence Name 35-K017629-022-053-G03C-AE01
GenBank Accession LN501632 [GenBank]
Sequencing primer gene specific primer, AE01: 5'-TCTGTTAACGCGTACACATACCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K017629-022-053-G03C-ae01
ACTGCAATATCCTCTCTGATGCAAAACGCAGCGTTTTGTCTCGTTTTAGCGTCCTTACCG
TTCAAGAACCGTACGAGACAGTTAAGAGAAGCCGTTGATCCCATCTTGTTGAAACCTTCT
GATCGATTCAGAGGCAACCAAGATGATAGAACAAACAAGATTTCCTCCAATAAACGCATG
TGTCCATCACGCGCCGCCGCGAATCTTTCAAAACAAGAAGACAAAGCCAAAACGCTTCCG
TTTTGGACCAAACACTTCTTGTTCGTGTCCCCTCCTCTTCCCAAGTTCTTGATTTTGCTC
ACAATCTCCAAACACTCGGCATACTCCTCACGCGCCGCTGCGTTTTGCAATCTCCGACTT
ATCTCCACCGCATCACGCGGCGTCAACAGCACGCGCGGTGACTGAATCCGCTCTATCCTT
GATGGCGATCGGAGTTGAACTGGAATCTTGGTTTCCACGACGGAGATATCGCCGGAAATC
AATTGATTACGTCTCTTTGCTACTCCTCCTCTTGATCTCCAAGGTAACACCATTATCTCG
TTTTCCTTTTTGTTTTGTTGGTTTGGTTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
T
   
Sequence Name 39-K017629-022-053-G03C-8409
GenBank Accession LN503186 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K017629-022-053-G03C-8409
TAGATTTCCCGGACATGAAACCAAACCAACAAAACAAAAAGGAAAACGAGATAATGGTGT
TACCTTGGAGATCAAGAGGAGGAGTAGCAAAGAGACGTAATCAATTGATTTCCGGCGATA
TCTCCGTCGTGGAAACCAAGATTCCAGTTCAACTCCGATCGCCATCAAGGATAGAGCGGA
TTCAGTCACCGCGCGTGCTGTTGACGCCGCGTGATGCGGTGGAGATAAGTCGGAGATTGC
AAAACGCAGCGGCGCGTGAGGAGTATGCCGAGTGTTTGGAGATTGTGAGCAAAATCAAGA
ACTTGGGAAGAGGAGGGGACACGAACAAGAAGTGTTTGGTCCAAAACGGAAGCGTTTTGG
CTTTGTCTTCTTGTTTTGAAAGATTCGCGGCGGCGCGTGATGGACACATGCGTTTATTGG
AGGAAATCTTGTTTGTTCTATCATCTTGGTTGCCTCTGAATCGATCAGAAGGTTTCAACA
AGATGGGATCAACGGCTTCTCTTAACTGTCTCGTACGGTTCTTGAACGGTAAGGACGCTA
AAACGAGACAAAACGCTGCGTTTTGCATCAGAGAGGTTATTGCAGTAGACAAGAGGTATG
TGT

Last Updated on 06.12.2018 16:11