Sequences from confirmation amplicon


Confirmation sequences for line 053G03 and hit At5g01840 are shown.
Line ID 053G03
Locus At5g01840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:324241
Primers used for PCR PH76 - 3144, show primer details
   
Sequence Name 27-K017511-022-053-G03C-PH76
GenBank Accession LN498168 [GenBank]
Sequencing primer gene specific primer, PH76: 5'-AAAACCAAGCGTTAGGGATTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K017511-022-053-G03C-PH76
CCGATAGTTATTACCCATTATCTCTCTGACTCTCTCTGTTGATTATTTTGTGTTTGTTGG
GGACTTCTTTGGTACATGAACAAAAGTGAGAGTGTGAAGAGTGGAGAATTTATAAAGGAA
AGAGAGGTTTATGTGAGGGGGCCCACATACGTGGAAAATATAAAGGTTATTATAAGGTGA
GTGAGATGCTCTCTCCATTTTCATTGATTTTGTGTTAGATTATGTTGGCCATTTCATTTT
CCCCTAAATTAAATTACATTGACATTTGACACTGACACTTTTATTATATGTTTTAGTAAT
AGTAATACACAAGTAGTGTTCGTACCAAAAACCGATAAATGATAGTTTAAACCGAAGGCG
GGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTAT
ATAAAAGAAGGGTCTTGCCAAAGATAATGGGATTGTGCGTCATCCCTTACCTCAGTG
   
Sequence Name 31-K017511-022-053-G03C-3144
GenBank Accession LN499949 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K017511-022-053-G03C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCATTTATCGGTTTTTGGTACGAACACTACTTGTGTATTACTATTACTAAAACATAT
AATAAAAGTGTCAGCGTCAAATGTCAATGTAATTTAATTTAGGGGAAAATGAAATGGCCA
ACATAATCTAACACAAAATCAATGAAAATGGAGAGAGCATCTCACTCACCTTATAATAAC
CTTTATATTTTCCACGTATGTGGGCCCCCTCACATAAACCTCTCTTTCCTTTATAAATTC
TCCACTCTTCACACTCTCACTTTTGTTCATGTACCAAAGAAGTCCCCAACAAACACAAAA
TAATCAACAGAGAGAGTCAGAGAGATAATGGGTAATAACTATCGGTTTAAGCTATCGGAG
TTAATCCCTA

Last Updated on 06.12.2018 16:11