Sequences from confirmation amplicon


Confirmation sequences for line 053G08 and hit At1g18150 are shown.
Line ID 053G08
Locus At1g18150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6245118
Primers used for PCR DL94 - 3144, show primer details
   
Sequence Name 61-K019284-022-053-G08C-3144
GenBank Accession LN510398 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K019284-022-053-G08C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTCAT
GAAGACATTACCATTTGGAGCATGATGTCTGTGAAGTGCAGTACTTCTTCCTCCTCCTGC
TGCTCCTGGTTTACCTTGATTTTCTTCAAGATGAGCAAATTGCCTTTTAAATCGGTCTAC
CCCACTGCGCGTAAACAACAAAAAGATTTATCGAGTTCATAACTTTCTGTTTTCAAGACT
TATAGTTTCTGTTAACTGTTAATTAGCTAACCTTGGGTACATAAAGCTAAGCTGATCACC
ACCACGAAGGTATTCCTCCAACATCTGAGGATGATATTCCAATATCTGATATTTACAAAA
TCCAAAGAAAAGTGATTGTTAGGATCAATGAATAACATTGAAACAAATTCAGATTAAAGA
AAAGGATATATGTAGAAGGCTTTTGTGTTTGTTTGTT
   
Sequence Name 35-K019284-022-053-G08C-DL94
GenBank Accession LN501641 [GenBank]
Sequencing primer gene specific primer, DL94: 5'-TTACCGAGAGGTAACAAACAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K019284-022-053-G08C-DL94
AACCTTCTCATATATCCTTTTCTTTAATCTGAATTTGTTTCAATGTTATTCATTGATCCT
AACAATCACTTTTCTTTGGATTTTGTAAATATCAGATATTGGAATATCATCCTCAGATGT
TGGAGGAATACCTTCGTGGTGGTGATCAGCTTAGCTTTATGTACCCAAGGTTAGCTAATT
AACAGTTAACAGAAACTATAAGTCTTGAAAACAGAAAGTTATGAACTCGATAAATCTTTT
TGTTGTTTACGCGCAGTGGGGTAGACCGATTTAAAAGGCAATTTGCTCATCTTGAAGAAA
ATCAAGGTAAACCAGGAGCAGCAGGAGGAGGAAGAAGTACTGCACTTCACAGACATCATG
CTCCAAATGGTAATGTCTTCATGAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGT
ACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGAT
AGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTG
AAGACGTGGTTGGAACGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGGG
Primers used for PCR FI20 - 8474, show primer details
   
Sequence Name 20-K028096-022-053-G08C-8409
GenBank Accession LN495072 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028096-022-053-G08C-8409
CTTTCTCTATTGATTGATGTTGCAGAGAGAGAGTTTCTCGCTTCAAATGGTGAAACTGCA
GAAGAAAGCAGTGATGTTTGAGAGAAGAGCAGCAGCTGCTGTGGCTTCAACCTTTGGAAT
CCGAGGAAGCAGAACAATGGAGGAGGTTACAGTGCTCGTAACCTCATGAAGAGTGCAAGC
ATCAGCGGTTCTAAATGCATTGGTGTCCAATCTAAAACTGACAAAGAGGTTAGTTAGTAA
AGAAAGAAAGAAAGAAAAACAGAAGATCTTGAAAAGGAACATGA
   
Sequence Name 20-K028096-022-053-G08C-FI20
GenBank Accession LN495073 [GenBank]
Sequencing primer gene specific primer, FI20: 5'-TAAGCAAACTTCATCGTCATGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K028096-022-053-G08C-FI20
TTCTTTCTTTACTAACTAACCTCTTTGTCAGTTTTAGATTGGACACCAATGCATTTAGAA
CCGCTGATGCTTGCACTCTTCATGAGGTTACGAGCACTGTAACCTCCTCCATTGTCTGCT
TCCTCGGATTCCAAGGTTGAAGCCACAGCAGCTGCTGCTCTTCTCTCAACATCACTGCTT
TCTTCTGCAGTTTCACCATTTGGAGCAGGAACTCTCTCTCTGCAACATCATCATAGAGAA
GCACAATGCTCTCAGAGGGAGATTGAGAAGAGAAGAGAAGAGATAGAGAGATTCTGTGAA
AGACATTACCTCCTCCTGTAAATGGCTTCATGACCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATT
ACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTT
ATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11