Sequences from confirmation amplicon


Confirmation sequences for line 053G11 and hit At1g43160 are shown.
Line ID 053G11
Locus At1g43160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16263937
Primers used for PCR WD05 - 8474, show primer details
   
Sequence Name 19-K016842-022-053-G11C-8409
GenBank Accession LN494546 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K016842-022-053-G11C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATGATTCTCTTTCCGATACAATATTAT
CCCCCGGGTTACAACTTGTCTACCACCGCAATTTCAAACATGGTGTCTATGCTGACTAAT
GTTGTCTCTGGTGAGACCGAACCCTCGGCATCTGCGACATGGACGATGGGTCATAAGAGA
GAAAGAGAAGAGTTTTCTTTGCCTCCTCAACCATTGATTACCGGTTCAGCTGGTATGCAT
GTTTTTCTAAATCGAATTTTTTTTTGTTGAGAACACTATACTAACCAATAAAACATTCTT
TAACCAAAAACAAAAATCAATAAAACAGTGACTAAAGAATGTGAAAGCTCAATGTCCTTG
GAGAGGCCAAAAAAATATAGAGGAGTAAGGCAACGACCATGGGGAAAATGGGCGGCGGAG
ATTCGAGACCCACACAAGGCGACACGTGTATGGCTTGGGACATTCGAGACAGCCGAGGCC
GCCGCAAGAGCCTATGATGCGGCAGCACTTCGCTTTAGAGGAAGCAAAGCAAAGCTTAAT
TTCCCCGAAAATGTTGGAACTCAGACGATTCAACGAAATTCTCATTTCTTGCAAAACTCT
ATGCAACCTTCTCTG
   
Sequence Name 20-K016842-022-053-G11C-WD05
GenBank Accession LN494998 [GenBank]
Sequencing primer gene specific primer, WD05: 5'-TTGATCGATGTATGTCAGAGAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K016842-022-053-G11C-wd05
AGTTTTGCAAGAAATGAGAATTTCGTTGAATCGTCTGAGTTCCAACATTTTCGGGGAAAT
TAAGCTTTGCTTTGCTTCCTCTAAAGCGAAGTGCTGCCGCATCATAGGCTCTTGCGGCGG
CCTCGGCTGTCTCGAATGTCCCAAGCCATACACGTGTCGCCTTGTGTGGGTCTCGAATCT
CCGCCGCCCATTTTCCCCATGGTCGTTGCCTTACTCCTCTATATTTTTTTGGCCTCTCCA
AGGACATTGAGCTTTCACATTCTTTAGTCACTGTTTTATTGATTTTTGTTTTTGGTTAAA
GAATGTTTTATTGGTTAGTATAGTGTTCTCAACAAAAAAAAATTCGATTTAGAAAAACAT
GCATACCAGCTGAACCGGTAATCAATGGTTGAGGAGGCAAAGAAAACTCTTCTCTTTCTC
TCTTATGACCCATCGTCCATGTCGCAGATGCCGAGGGTTCGGTCTCACCAGAGACAACAT
TAGTCAGCATAGACACCATGTTTGAAATTGCGGTGGTAGACAAGTTGTAACCCGGGGGAT
AATATTGTATCGGAAAGAGAATCATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTG
ATAAA
Primers used for PCR FM18 - 3144, show primer details
   
Sequence Name 37-K029300-022-053-G11C-3144
GenBank Accession LN502496 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K029300-022-053-G11C-3144
ATCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGA
CAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGA
CCATCATACTCGTTTATGATACTCGTTATTTCTTCGGCATTGTAGTGATACTGGCCATTG
CCGGTTTCTTCGTTAGAGTCACGTTGGTTCGCCAGTTTTGTGTTGCTCAGTGTAATCAAA
TTGTGTTGTTCCTATCTTTTTAGTAGATGCTTGCTTATATAATCCGGAGTCACTAGTTAA
TTCAACATGTCTTTCTCTAGCCGTCATACAAATAGTGACACGTGGGAAATGGACATAAAC
AAAAGATTTCCTTACCAACTTGAATTAGACCTTCATGACACGTTGATTGATTTAGCCACA
CAAATATATTATAAATTTTAAATATTTATTTCGGTTTTGTTTTTATATATGTAGTAACAA
AACTAGCTAGACTTAGTTAAAGTTTTTTTTTTTGTTGGTCAACTAGTTAAAGACTCAAAG
ATGTAATATTTATGTTATAAGGCATGTAATGAAATATGAAACACGTGTTGAAATATTCAA
AAACGTGCGTTCACATTCAGGCTTAGAAATCGAAGTA
   
Sequence Name 38-K029300-022-053-G11C-FM18
GenBank Accession LN502867 [GenBank]
Sequencing primer gene specific primer, FM18: 5'-GGGTTCACAAAATTGTACTTCGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K029300-022-053-G11C-FM18
TTTTTGAAATTTCAACACGTGNTTTCATATTTCATTACATGCATTATAACATAAATATTA
CATCTTTGAGTCTTTAACTAGTTGACCAACAAAAAAAAAAACTTTAACTAAGTCTAGCTA
GTTTTGTTACTACATATATAAAAACAAAACCGAAATAAATATTTAAAATTTATAATATAT
TTGTGTGGCTAAATCAATCAACGTGTCATGAAGGTCTAATTCAAGTTGGTAAGGAAATCT
TTTGTTTATGTCCATTTCCCACGTGTCACTATTTGTATGACGGCTAGAGAAAGACATGTT
GAATTAACTAGTGACTCCGGATTATATAAGCAAGCATCTACTAAAAAGATAGGAACAACA
CATTTTGATTACACTGAGCAACACAAAACTGGCGAACCAACGTGACTCTAACGAAAAAAC
CGGCAATGGCCAGTATCACTACAATGCCGAAAAAATAACGAGTATCATAAACGAGTATGA
TGGTCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACC
CTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGT
GGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAG
ACGTGGTTGGAACGTCTTCTTTTTCCACAATGCTCCTCGTG

Last Updated on 06.12.2018 16:11