Sequences from confirmation amplicon


Confirmation sequences for line 054E12 and hit At3g25710 are shown.
Line ID 054E12
Locus At3g25710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9370101
Primers used for PCR H32a - 8474, show primer details
   
Sequence Name 30-K014430-0022-054-E12C-8409
GenBank Accession LN499504 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K014430-0022-054-E12C-8409
TCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATACACTCTTCTCCTTTC
ATTATGGTTTGATATTTCAGAAACTAATTCACTATTTTGAAAACCCTATGGAAAAATAGT
TTTAATTAATTAATGATCCTTCAAAATTTCTCTTTATTTAGTTATTATTGGTTAGAGTAG
TAGTAATTGTTGTTGTTTTTTAATAAAGAGAGTAGTAGTAATTGTTCTTTTTCTGATAAA
TATAGTACTAATTATTCAGTTTCAAAAATGATAAGTACTATATTTTTCAATATTATCATT
TTTATCTATCTCTGTTCAACTATTATCGTTTAGTAGTTGTAAATTTTCAAATTTAAAATA
AATGTATCAAATCGATATTTTTGGTAAGTAATTATAGTCCACAGATGGGGTATTTTTGTA
CTAGTTATTATTATTGAAGAAGAAGAATAAGAAGATTAAATACTTATGATTCGTTTTGGT
TTTGTATAGACGGACAAAGCTTCTTTGCTAGCGGAAGTGATCCAACACATGAAGGAGCTA
AAACGACAAACATCACAGATCACCGACACGTATCAAGTCCCAACAGAGTGCGATGATCTG
ACCGTAGATTCGTCTTACAACGACGAGGAAGGAAACTTGGTGATAAGAGCATCCTTTTGC
TGCCAAGACAGGACTGACCTCATGCATGACGTCATCAATGCCTTAAAGTCTCTTCGTCTT
CGAACTCTCAAAGCTGAGATCGCAACCGTAGGTGGT
   
Sequence Name 31-K014430-022-054-E12C-H32a
GenBank Accession LN499936 [GenBank]
Sequencing primer gene specific primer, H32a: 5'-ATCCTCCACGTCATCACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K014430-022-054-E12C-foo
GTTTCTACGATATGAATCATGATCTTCTTCATCATCGTATTCTCGGCTCAAGAACAAGAT
GTTCTTGACTCTACCACCTACGGTTGCGATCTCAGCTTTGAGAGTTCGAAGACGAAGAGA
CTTTAAGGCATTGATGACGTCATGCATGAGGTCAGTCCTGTCTTGGCAGCAAAAGGATGC
TCTTATCACCAAGTTTCCTTCCTCGTCGTTGTAAGACGAATCTACGGTCAGATCATCGCA
CTCTGTTGGGACTTGATACGTGTCGGTGATCTGTGATGTTTGTCGTTTTAGCTCCTTCAT
GTGTTGGATCACTTCCGCTAGCAAAGAAGCTTTGTCCGTCTATACAAAACCAAAACGAAT
CATAAGTATTTAATCTTCTTATTCTTCTTCTTCAATAATAATAACTAGTACAAAAATACC
CCATCTGTGGACTATAATTACTTACCAAAAATATCGATTTGATACATTTATTTTAAATTT
GAAAATTTACAACTACTAAACGATAATAGTTGAACAGAGATAGATAAAAATGATAATATT
GAAAAATATAGTACTTATCATTTTTGAAACTGAATAATTAGTACTATATTTATCAGAAAA
AGAACAATTACTACTACTCTCTTTATTAAAAAACAACAACAATTACTACTACTCTAACCA
ATAATAACTAAATAAAGAGAAATTTTGAAGGATCATTAATTAATTAAAACTATTTTTCCA
TAGGGTTTTCAAAATAGTGAATTAGTTTCTGAAATATCAAACCATAAT

Last Updated on 06.12.2018 16:11